The present study aimed to investigate the possible roles of lecithin and quercetin either singly or combined against Ifosfamide-induced DNA damage in corneas. Healthy female Rattus norvegicus rats weighing 180 ±10 g were divided into seven groups each comprised of ten rats. Group I served as control and intraperitoneally injected with 0.25 ml of physiological saline (0.9% NaCl) for 5 days. Groups II and III were orally administered lecithin at a dose of 100 mg/kg body weight or quercetin at a dose of 50 mg/kg body weight suspended in distilled water, respectively for six days; whereas group IV was used as positive control and intraperitoneally injected with Ifosfamide at a dose of 80mg/kg body weight for five days. Groups V and VI were injected with either quercetin or lecithin along with Ifosfamide by the same treatment regimens, respectively. Group VII received a combination of both protective agents along with Ifosfamide and treated similarly. Corneas from all groups were used for comet assay. Results: The lecithin and quercetin treated groups were characterized by normal comet parameters compared to control. After Ifosfamide injection, all comet parameters were significantly increased (p˂0.05). No changes in comet parameters were observed for lecithin-Ifosfamide treated group as well as for quercetin-Ifosfamide one. Co-administration of lecithin and quercetin indicate significant increase (p˂0.05) in all comet parameters (group VII). All together led to conclude that Ifosfamide causes DNA damage of rat cornea due to its oxidative stress and, co-administration of lecithin and quercetin was contradicting each other while using these antioxidants separately has beneficial effects on corneal genetic material.
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