The relationship between orexin/hypocretin and rapid eye movement (REM) sleep remains elusive. Here, we find that a proportion of orexin neurons project to the sublaterodorsal tegmental nucleus (SLD) and exhibit REM sleep-related activation. In SLD, orexin directly excites orexin receptor-positive neurons (occupying ~3/4 of total-population) and increases gap junction conductance among neurons. Their interaction spreads the orexin-elicited partial-excitation to activate SLD network globally. Besides, the activated SLD network exhibits increased probability of synchronized firings. This synchronized excitation promotes the correspondence between SLD and its downstream target to enhance SLD output. Using optogenetics and fiber-photometry, we consequently find that orexin-enhanced SLD output prolongs REM sleep episodes through consolidating brain state activation/muscle tone inhibition. After chemogenetic silencing of SLD orexin signaling, a ~17% reduction of REM sleep amounts and disruptions of REM sleep muscle atonia are observed. These findings reveal a stabilization role of orexin in REM sleep.
Ecological well-being performance (EWP) is central to achieving coordinated and sustainable economic and social development and environmental protection. This study constructed an evaluation index system of EWP, measured the EWP of 30 Chinese provinces or cities from 1997 to 2018 using the super-efficiency slack-based model (Super-SBM), and analyzed the spatial and temporal evolutionary characteristics of EWP. Under the division of four regions and eight regions, the Dagum Gini coefficient decomposition is applied to analyze the regional differences and sources of differences in EWP in China. Then, the Geographically and Temporally Weighted Regression (GTWR) model analyzes the factors influencing EWP. Results show that the inter-provincial differences in EWP in China are significant, with the eastern region having significantly higher EWP than the western, central, and northeastern regions. From 1997 to 2018, the overall spatial differences in EWP in China decreased. The four regions and eight regions show that reducing inter-regional differences is the key to mitigating regional unbalance in China. Urbanization significantly enhances EWP in China and the degree of openness and industrial structure has a significant heterogeneous effect on EWP. Therefore, future policy formulation should focus on transforming the economic development model, promoting coordinated regional development, and exploring the optimal ways to improve EWP according to local conditions. This study aims to provide a scientific basis and reference for promoting sustainable regional economic and social development and improving the imbalance.
scite is a Brooklyn-based organization that helps researchers better discover and understand research articles through Smart Citations–citations that display the context of the citation and describe whether the article provides supporting or contrasting evidence. scite is used by students and researchers from around the world and is funded in part by the National Science Foundation and the National Institute on Drug Abuse of the National Institutes of Health.