The herbicide chlorimuron-ethyl has been applied widely for weed control in farmland, especially in soybean fields in China over the past decade, but the chronic effects of this herbicide on soil microorganisms, particularly Pseudomonas spp., is not well understood. Taking a continuously cropped soybean field in the town of Fuyuan-a soybean production base of Heilongjiang Province in Northeast China-as a case study, soil samples were collected from plots having received 0-, 5-, and 10-year applications of chlorimuron-ethyl (30 g active component of chlorimuronethyl/ha/year) to study the abundance and diversity of Pseudomonas spp. Meanwhile, an in vitro assay was used to examine the antifungal activities of isolated Pseudomonas spp. against soil-borne pathogens (Fusarium graminearum, Fusarium oxysporum, and Rhizoctonia solani) causing soybean root rot disease. The production of siderophore, hydrogen cyanide (HCN), and lytic enzymes (cellulase, pectinase, and chitinase) by Pseudomonas spp. was also investigated. With 5-and 10-year chlorimuronethyl application, the numbers of soil Pseudomonas spp. decreased from 121×10 2 CFU/g dry soil in the control to 40×10 2 CFU/g dry soil and 13×10 2 CFU/g dry soil, and the Shannon index values decreased from 6.23 to 3.71 and 1.73, respectively. The numbers of antifungal Pseudomonas spp. also decreased, and the proportions of Pseudomonas spp. with antifungal activities against the different test pathogens altered. All the antifungal Pseudomonas spp. could produce siderophore and HCN but not lytic enzymes. The results suggest that long-term application of chlorimuron-ethyl in continuously cropped soybean field had negative effects on the abundance and diversity of soil Pseudomonas spp., including species with different antifungal activities against pathogens. Siderophore and HCN rather than lytic enzymes formed the antifungal metabolites of Pseudomonas spp., and the number of antifungal Pseudomonas that can produce siderophore and HCN decreased markedly under application of chlorimuron-ethyl, especially after 10-year application.
Maintaining high system performance is critical for a multi-hop flying mesh network (FlyMesh) to perform missions in different environments. Although the Poisson point process (PPP) has been widely used for the performance analysis of FlyMesh, it still has flaws in describing the spatial distribution of the UAVs since it does not restrict the minimum distance between them. The spatial deployment of FlyMesh varies depending on the environment. Considering the relevance and practicality, we modeled the multi-hop FlyMesh using the β-Ginibre point process (β-GPP) and equipped each UAV with a directional antenna. Under the condition of the decode-and-forward protocol, we derived the connection probability and ergodic capacity of a multi-hop FlyMesh utilizing the Laplace transform of interference. Then, we calculated an approximate expression for the interference Laplace transform based on the diagonal approximation and further obtained the coverage probability. Finally, the numerical simulation results verified the correctness of the theoretical derivation, indicating that it is possible to optimize the system’s performance based on the expressions derived in this paper.
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