Anthocyanins from the purple Solanum tuberosum newly cultivated by the Taian Academy of Agricultural Sciences were extracted and analyzed using high-performance liquid chromatography (HPLC) and UV-vis spectroscopy. Four individual anthocyanins were detected as the major components, and the total anthocyanin content was 273.5 ± 14.3 mg of cyanidin-3-glucoside equiv/100 g of dry seeds. Results of color stability showed that the purple S. tuberosum anthocyanins (PSTAs) are more stable under low pH and temperatures. Heat and general food additives have fine compatibility with PSTAs; however, they are very sensitive with oxidant and reduction. The influence of PSTAs on Cr(VI) targeted to bovine serum albumin (BSA) was also studied. The quenching of BSA fluorescence caused by Cr(VI) could be delayed by PSTAs. UV-vis and circular dichroism (CD) data suggested that PSTAs can protect the secondary and tertiary structures of BSA by probably interacting with Cr(VI) in advance.

Purple pigments were isolated from mulberry extracts using preparative high-speed countercurrent chromatography (HSCCC) and identified by ESI-MS/MS and high performance liquid chromatography (HPLC) techniques. The solvent system containing methyl tert-butyl ether, 1-butanol, acetonitrile, water, and trifluoroacetic acid (10:30:10:50:0.05; %, v/v) was developed in order to separate anthocyanins with different polarities. Cyanidin 3-O-(6″-O-α-rhamnopyranosyl-β-galactopyranoside) (also known as keracyanin) is the major component present in mulberry (41.3%). Other isolated pigments are cyanidin 3-O-(6″-O-α-rhamnopyranosyl-β-glucopyranoside) and petunidin 3-O-β-glucopyranoside. The binding characteristics of keracyanin with human serum albumin (HSA) were investigated by fluorescence and circular dichroism (CD) spectroscopy. Spectroscopic analysis reveals that HSA fluorescence quenched by keracyanin follows a static mode. Binding of keracyanin to HSA mainly depends on van der Waals force or H-bonds with average binding distance of 2.82 nm. The results from synchronous fluorescence, three-dimensional fluorescence, and CD spectra show that adaptive structure rearrangement and decrease of α-helical structure occur in the presence of keracyanin.

No abstract

In this work, the interaction of chlortetracycline with bovine serum albumin (BSA) was investigated by fluorescence spectroscopy, circular dichroism (CD) spectroscopy, and molecular docking. Results indicated that chlortetracycline quenches BSA fluorescence mainly by a static quenching mechanism. The quenching constants (KSV) were obtained as 5.64 × 104, 4.49 × 104/, and 3.44 × 104/ M−1 at 283, 295, and 307 K, respectively. The thermodynamic parameters of enthalpy change Δ H°, entropy change Δ S°, and free energy change Δ G° were −5.12 × 104/ J mol−1, −97.6 J mol−1 K−1, and −2.24 × 104/ J mol−1 (295 K), respectively. The association constant (KA) and the number of binding sites (n) were 9.41 × 103/ M−1 and 0.86, respectively. The analysis results suggested that the interaction was spontaneous, and van der Waals force and hydrogen‐bonding interactions played key roles in the reaction process. In addition, CD spectra proved secondary structure alteration of BSA in the presence of chlortetracycline. © 2012 Wiley Periodicals, Inc. J Biochem Mol Toxicol 26:331–336, 2012; View this article online at wileyonlinelibrary.com. DOI 10:1002/jbt.21424

With the development of my country’s economy and society and the improvement of civilization, the silver wave has gradually come. The country has launched a multifaceted pension model, such as institutional pension, home-based pension, and pension real estate. With the increasing aging of the population, traditional elderly care services can no longer meet the growing needs of the elderly. This research mainly discusses the construction of a network information security platform based on artificial intelligence for the elderly’s health “integration of physical, medical, and nursing care.” The platform consists of five modules: health records, follow-up plans, remote training, health education, and remote consultation. Each module is equipped with a corresponding main interface and/or subinterface. Some modules also have submodules as needed. The structure is reasonable, and the interface is displayed. The combined medical care service model is divided into medical care, care care, and medical care. Among them, the medical care model mainly provides long-term comprehensive care services for the disabled, demented, semidisabled, and other elderly groups. The support-oriented model is mainly for self-care or semi-self-care elderly groups, providing rehabilitation monitoring and life care services. In terms of the overall effect of the platform, 13 users (81.25%) gave a high evaluation of the overall effect of the platform. This research will promote the development of the smart elderly care industry.

With the increasingly serious population aging, economic system transformation, and social transformation, the elderly have an increasingly strong demand for the elderly care service industry, but the development of China’s elderly care service industry started late, and there are still many problems. For how to build a better elderly care service model, under the current situation of shortage of elderly care resources, community elderly care may become a solution. By analyzing the status quo, existing problems and development trends of home-based care services in Hangzhou’s community, and learning from foreign experience, it explores the establishment of a “integrated physical, medical, and nursing care” community health management model for the elderly and provides a perfect solution for the development of Hangzhou’s community elderly care services countermeasures and suggestions for mode operation. Based on the evaluation, problem analysis, and empirical research on the current situation of home care services in Hangzhou communities, this paper draws on relatively mature community care practices, proposes to build a community elderly health management model that combines medical care and care, and improves and innovates the overall design and medical care of the elderly service model measures for the health management of the elderly in the integrated community. The experimental results of this study show that, according to the demand for elderly care services, elderly people in need of care account for 86.6%, 79.5%, and 68.4% of the elderly population, especially in the areas of medical care, life care, and housekeeping services.

Abstract-Animation demonstration is an important manifestation of multimedia technology. In the past, low flexibility and long research and development cycle are typical problems of animation demonstration system and video conversion technology. Through introducing an advanced teaching method (understanding approach) and multimedia technology (video conversion system based on SOPC) for theoretical construction, this study designed a video conversion system based on SOPC, finally constructed a multifunctional video conversion-based multimedia teaching system from the aspect of module design and functional design, and used a randomized controlled experiment to test students' learning outcomes in the course of college basketball. The results show that students' offensive understanding ability and defensive understanding ability improved greatly after the system was used in teaching. It plays an important role in improving the usability and effectiveness of multimedia teaching system.

Traditional basketball teaching has some problems, including the duplication of contents, outdated methods, disconnection between theories and practice, and lack of discussion on the use of computer technology and Internet technology to optimize the existing teaching mode. Based on the theoretical basis of “learning activities as the center”, a LAMS (Learning Activity Management System) teaching mode was proposed in this study for the course of “College Basketball”. It consists of three parts: the design module, the learning module, and the monitoring module. The LAMS-based college basketball teaching mode mainly includes learning activities, and learning resources and tools. Learning activities mainly involve teaching objectives, creation of learning activities, collection of information concerning learning activities, information analysis and improvement of activities. Meanwhile, learning resources and tools were set for the smooth progress of learning activities. After the teaching mode was constructed, it was applied in practical teaching at Shandong Normal University in Shandong Province, China. The teaching results show that compared to the traditional teaching mode, the LAMS teaching mode centered on learning activities can significantly improve the effect of teaching College Basketball.