Tiny marine animals represent an untapped reservoir for undiscovered, bioactive natural products. However, their small size and extreme chemical variability preclude traditional chemical approaches to discovering new bioactive compounds. Here, we use a metagenomic method to directly discover and rapidly access cyanobactin class natural products from these variable samples, providing proof-of-concept for genome based discovery and supply of marine natural products. We also address practical optimization of complex, multistep ribosomal peptide pathways in heterologous hosts, which is still very challenging. The resulting methods and concepts will be applicable to ribosomal peptide and other biosynthetic pathways.
Abstract. Specifying service-oriented system, which is a new type of Enterprise Information System, is critical with the rapid development of Web Services. For the purpose, this paper presents an approach to model ServiceOriented Architecture (SOA). We do not exclude the traditional opinions of software architecture description as the current specification approaches; on the contrary, some similar conceptions, such as service components and connectors are defined in this paper. For architectural reuse, the notion of composite service components is specified to model services composition and that of composite connectors is defined to abstract the complex communication protocols. Within the context of the Travel Reservation System (TRS), we demonstrate the usage and practicability of our approach based-on Web Services.
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