Infection with Fasciola gigantica, a liver trematode, is frequently reported in Malang district. The study was aimed to determine the prevalence of fascioliasis in Malang District, East Java during Eid Qurban 2019. Data were collected from 68 cutting points in 9 sub-districts. A total of 265 cows, 895 goats and 112 sheep than analyzed based on questioner emphasis on age, origin, body condition, and postmortem abnormalities. Overall the highest prevalence of fasciolosis was 57% cattle and 18% goat in Gondanglegi sub-district and 100% sheep in Wajak sub-district. A total of 86% of fasciolosis in cattle have good body conditions came from Malang District. There was also 82% in goat and 78% in sheep infected came from Malang District too. Morphological and postmortem examination findings that 53% of adult cattle (2-3 years), adult goat and sheep (1-1,5 years) were 78% and 89% respectively have abnormalities in the liver. This data showed adult livestock more resistant to fasciolosis infection. From that data above that further surveys should be carried out to understanding on the epidemiology of fascioliasis in the study area and classified risk factors affecting the infection.
The aim of this research was to explore the influence of Mozart KV 448 classical music therapy to the neuroglia cells of mice's hippocampus that were exposed to stress during prenatal. This research were employing twenty female mices and twenty male mices. Female mices were estrus synchronized with PMSG and hCG then monomating to the males. Pregnant females were then divided into four groups (P0, P1, P2, P3). P0 was as control, P1 was treated by one minute forced swim test, P2 was treated by one minute forced swim test followed by thirty minutes classical music Mozart KV 448 and P3 was treated by one minute forced swim test followed by sixty minutes classical music Mozart KV 448. This research was carried out for twenty-one days during gestation period. The neuroglia density result was analyzed using ANOVA and Duncan test. The neuroglia activity result was analyzed using Kruskal wallis test and Z test. The histology reading showed degradation of density and activity of hippocampus neuroglia.Keywords: prenatal stress, classical music, Mozart KV 448, neuroglia, hippocampus AbstrakTujuan dari penelitian ini adalah untuk mengetahui pengaruh terapi musik klasik Mozart KV 448 pada densitas dan aktivitas dari sel neuroglia hipokampus anak mencit yang terpapar stres selama prenatal. Penelitian ini menggunakan 20 mencit betina dan 20 mencit jantan. Mencit betina kemudian di sinkronisasi estrus dengan PMSG dan hCG setelah itu dilakukan mono mating. Mencit betina yang positif bunting di bagi menjadi empat kelompok (P0, P1, P2, P3). P0 sebagai kontrol tidak diberikan perlakuan apapun, P1 diberikan terapi forced swim test selama satu menit, P2 diberikan terapi forced swim test selama satu menit diikuti musik klasik Mozart KV 448 selama 30 menit dan P3 diberikan terapi forced swim test selama satu menit diikuti musik klasik Mozart KV 448 selama 60 menit. Penelitian ini dilakukan selama 21 hari periode kebuntingan. Analisis data pada densitas neuroglia menggunakan ANOVA dan uji Duncan selanjutnya untuk aktivitas neuroglia data yang diperoleh berupa skor nilai tingkat perubahan aktivitas antar neuroglia sehingga menggunakan uji Kruskal wallis dan uji Z. Hasil gambaran histologi dari penelitian ini menunjukkan penurunan terhadap densitas dan aktivitas neuroglia hipokampus.
As an annual Muslim celebration, Eid al-Adha Feast is celebrated every year in Malang City. In 2019 feast, animals were sacrificed in Malang City consisting of 1202 cattle, 3572 goats and 302 sheep. Antemortem and postmortem examination were very important steps to ensure the quality of consumable animal product produced during the feast. The importance of these examination was to prevent zoonotic food-borne and transmissible disease from contaminated animal product to other livestocks nearby. Fascioliosis was listed into routine disease check due to its potential to reduce the quality of animal product, especially the liver. This study was held in 5 sub-districts in Malang City according to descriptive data collected by interview and structural questionnaire as well as visual observation. The result showed that fascioliosis happened in 26,03% of cattle, 2,93% of goats, and 1,32% of sheep being slaughtered. We also analized several risk factors influencing fascioliosis incidence rate such as body score condition and age. The fascioliosis tent to happen in medium and mature animal by age in respectively 31,62% of cattle, 54,28% of goats, and 75% of sheep. Thus, prevention and curative evaluation of fascioliosis should be continuously developed in order to provide excellent animal products.
Eimeria is a genus of apicomplexan parasites that infected various species and known as the enteric monoxenous coccidian. This study aims to determine the prevalence, identification and geographical distribution study of gastrointestinal protozoa Eimeria spp. in wild rodents in Malang city, East Java. A total of 74 rodents were collected from different part of Malang, using single live traps. Rats were euthanized with ketamine and xylazine. Samples of stools were taken from gastrointestinal tract. Coprological procedure using floatation method lead to the demonstration of Eimeria spp. oocysts in the faecal samples examined at 400 and 1000 magnifications of light microscope. Cultivation in dichromate potassium 2.5% was carried out for all of coccidian positive samples. The identification is based on morphology and morphometry. Eimeria identification data was analyzed descriptively, while the analysis of species and gender of rodents on prevalence used chi square. Result, the prevalence of Eimeria spp. in Malang is 11 (14.9%). It was suggesting coccidiosis caused by Eimeria nieschulzi. In addition, Chi square analysis showed that species of rats did not have a significant relationship (P>0.05) to the incidence of Eimeria infection, however it has significant correlation between gender of rats and Eimeria infection (P<0.05). The geographical of Eimeria spp. spots were showed in 8 Sampling area, in Lowokwaru, Klojen, Kedung kandang and Sukun sub district. Findings of this study showed that wild rodents in Malang city are infected with intestinal coccidian parasites.
Strongyloidiasis is a nematode parasite with broader distribution proved to infect humans and animals. Strongyloides ratti common endoparasites infected rodent as the most adaptive population in various environments. We have currently raised concerning the neglected tropical diseases (NTDs) situations in Indonesia. This study aimed to understand the incidence of Strongyloides spp. infecting wild rodents in Malang City during October to December 2021 by stratified random sampling. A total of 50 rats (Rattus norvegicus, Rattus rattus diardii, Mus musculus), 26 male (52%) and 24 female (48%). Following 12 juveniles (24%) and 38 adults (76%). Coprology examinations use floatation and sedimentation methods immediately after the gastrointestinal tract's stool collection (GITs). We also administered a limited survey to get responses from 80 people (housewives, sellers, employee, and students) to obtain risk transmission, public awareness, and attitudes. We were continually analyzed data using the chi-squared and Fisher Exact Test. The microscopic examination of stools was 28% positively detected S. ratti. Our study found an association presented between the age of rats and Strongyloidiasis infections (p<0.05). However, the gender of rats did not have a significant association (p>0.05) to the S. ratti several infections. In addition, most participants did not understand the zoonotic disease and these infections, indicating a low-level knowledge 71 (88%), because better education supports the increase of awareness. However, almost all participants have good practice of hygiene and sanitation toward COVID-19 situations 72 (90%). The further study recommends investigating Strongyloidiasis infections in another species and increasing the education program for housewives and sellers in a traditional marketplace to have better knowledge.
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