Fall application of anhydrous ammonia (NH3) is a common practice for corn (Zea mays L.) production in the midwestern United States, but evaluations to date have relied entirely on yield comparisons that provide no means of distinguishing fertilizer from soil N uptake. To quantify fertilizer N uptake efficiency (FNUE) when using this practice, field trials using 15NH3 were conducted between 2016 and 2018 at four sites in a corn–soybean (Glycine max L. Merr.) rotation and at two sites under continuous corn. At each site, 224 kg N ha−1 was applied with and without the use of nitrapyrin (NP) to inhibit nitrification. Relative to grain yields without fertilizer N, response to fall N fertilization occurred at four of the six sites studied, averaging 53% (4.0 Mg ha−1) in two growing seasons with below‐normal rainfall. The use of NP was beneficial at only one site for increasing total N uptake, but resulted in a decrease at another, along with a significant reduction in grain yield. Isotopic estimates of FNUE (i.e., F15NUE) ranged from 12 to 34% (21% on average) for grain and from 16 to 42% (28% on average) for total aboveground biomass, and N derived from fertilizer ranged from 20 to 46% (32% on average). Both isotopic parameters were highest for the site lowest in native N availability, demonstrating the potential of site‐specific N management to improve fall NH3 fertilization by accounting for soil N mineralization.
Heirloom tomato varieties are in demand by consumers due to high antioxidant levels. However, these varieties are difficult to produce and are prone to disease. To overcome these problems, heirloom tomatoes may be cultivated in hydroponic systems and grafted onto disease-resistant rootstocks. However, it is unknown if the antioxidant content and capacity are affected by grafting. In this study, heirloom (Black Krim and Green Zebra) and standard (Big Beef) varieties were grafted onto wild type (WT) or productive rootstocks (Arnold and Supernatural). The tomatoes were harvested at maturity, freeze-dried, and ground into a powder. Lycopene was extracted using hexane, and the content was determined spectrophotometrically at 503 nm. The antioxidant capacity of methanol extracts was evaluated by the 2,2′-azino-di[3-ethylbenzthiazoline sulfonsyr]sulphonic acid (ABTS) and 2,2-diphenyl-1-picrylhydrazyl (DPPH) assays, whereas the phenolic content was determined using the Folin–Ciocalteu assay. Interestingly, the grafting of Big Beef and Green Zebra onto Supernatural rootstock resulted in an increased antioxidant capacity, as determined by the DPPH assay. Moreover, the phenolic content was changed for Big Beef grafted onto Arnold, and Big Beef and Green Zebra grafted onto Supernatural. Taken together, these results indicate that certain combinations of standard and heirloom tomato varieties and productive rootstocks may influence the antioxidant capacity and phenolic content. These results may be used to guide producers when choosing rootstocks for cultivating hydroponic tomatoes.
F oliar N fertilization is an increasingly common practice in the turfgrass industry. This trend is due to ease of application of foliar N fertilizers, their compatibility with other commonly applied turf care products, and the ability to uniformly apply low doses (<2 g m -2 ) of N to high-value areas of turf.Tracer and nontracer techniques have been used to estimate fertilizer N absorption by turfgrass leaves. The tracer approach uses the stable isotope, 15 N, to directly quantify plant uptake of labeled fertilizer N. The nontracer, or indirect, alternatives include the difference method and a washing technique. The difference method compares plant N concentration with and without fertilization and ascribes any increase for the fertilized treatment to fertilizer N uptake. The washing technique quantifies N uptake as the difference between the amount of fertilizer applied and the amount recovered in a foliar rinse.Indirect methods have been used in several studies to quantify foliar N uptake by turf. The earliest of these was reported by Wesely et al. (1985) for five cool-season turfgrasses maintained in a growth chamber. Their results, using the difference method, showed 31 to 61% uptake in the first 72 h after application, depending on species and cultivar. In a subsequent field study, Wesely et al. (1988) found 49 to 59% uptake by the difference method following repeated ABSTRACT Turfgrass managers frequently apply N as a foliar spray when low application rates are desired. This practice is believed to promote foliar N uptake that benefits turf; however, very little information is available concerning the quantity of N absorbed by turfgrass foliage or the effect of various spray parameters on foliar N uptake under field conditions. This research was conducted to evaluate fertilizer N uptake efficiency of foliarly applied 15 N to creeping bentgrass [Agrostis stolonifera var. L. palustris (Huds.) Farw. 'pennlinks'] under field conditions. The effects of spray volume, N carrier, adjuvant addition, and tank mixing with commonly applied turf care products (e.g. chlorothalonil) on foliar N uptake were conducted to evaluate foliar uptake of fertilizer 15 N by creeping bentgrass. From 6 to 34% of foliar-applied N was taken up mostly within 2 h and completely by 4 to 6 h after fertilizer application. Uptake efficiency increased significantly when spray volume was decreased but was unaffected by N carrier, adjuvant addition, or tank mixing.
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