The rates of endocytosis, intracellular degradation, and cell-surface shedding of 125I-labeled monoclonal antibodies (MoAbs) HD-37 (anti- CD19), B1 (anti-CD20), MB-1 (anti-CD37), BC8 (anti-CD45), and DA4–4 (anti-mu) by B-lymphoma cells were compared by cellular radioimmunoassay, ultrastructural autoradiography, sodium dodecyl sulfate-polyacrylamide gel electrophoresis, and thin layer chromatography using biopsy specimens from 12 patients with non- Hodgkin's lymphomas. 125I-BC8 was stably retained on the surface of lymphoma cells without appreciable internalization or shedding, whereas 125I-DA4–4 underwent rapid endocytosis and degradation. 125I-B1 was not internalized or degraded by tumor cells, but rapidly dissociated from the cell surface in intact form. Moderate rates of endocytosis, intracellular metabolism, and cell-surface shedding were shown by 125I- HD37 and 125I-MB-1. The 3 patients with diffuse, small cleaved-cell lymphomas internalized and degraded antibodies more slowly than did patients with other histologic subtypes. These kinetic differences may be important in the selection of MoAbs for immunotoxin and radioimmunoconjugate therapy of B-cell malignancies.
The late adoption of pottery technology in the North American Arctic between 2,500 and 2,800 years ago coincides with the development of a specialized maritime economy. Arctic pottery technologies present an excellent case study for examining possible correlations between hunter-gatherer pottery and aquatic resource use. Review of the timing and distribution of early pottery in Alaska shows that early pottery is rare and dates at the earliest to 2,500 years ago; the earliest pottery is found in small numbers and primarily in coastal areas. Despite expectations that pottery use would be strongly linked to marine lipids, biomarkers and compound-specific δ13C values of 20 sherds from the Cape Krusenstern site complex, dating from 2700 to 200 cal B.P. years ago, are most consistent with freshwater aquatic resources; mixtures of freshwater aquatic, marine aquatic, and terrestrial resources are also possible. While additional analysis of a larger sample and zooarchaeological reference specimens is necessary, our study suggests that the development of pottery production by Arctic peoples is more complex than previously appreciated. This research is the first synthesis in over 30 years of early pottery in Alaska and is the first to include residue analysis of a small sample of pre-1500 B.P. pottery.
a b s t r a c tThis research examines the evidence for prehistoric ceramic exchange networks over the last 2000 years in northwest Alaska through the use of neutron activation analysis of ceramic artifacts. Results from ceramic analysis on eight coastal and inland archaeological sites identified three source macrogroups and three associated subgroups. Clay source diversity and shared source macrogroups between geographically distant sites suggest the use of multiple sources and/or the movement of pots between production locales, mirroring related patterns in pottery stylistic data. Although additional analytical work is needed to fully understand the changing character of clay procurement and ceramic distribution across this time period, this study provides exploratory data on past ceramic production and distribution that hints at changes in exchange and territoriality in northwest Alaska during the late Holocene.
Why, when, and how people developed highly specialized marine economies remains the focus of considerable anthropological research. Study of maritime adaptations at high latitudes has potential to contribute to this debate because low biodiversity and increased resource seasonality at high latitudes made reliance on marine resources particularly risky. New research at the Cape Krusenstern site complex, located in northwest Alaska, offers a rare opportunity to study the evolution of maritime adaptations across the environmentally dynamic mid-to-late Holocene Arctic. Large-scale and systematic survey of this important site complex was undertaken to address questions about the timing and character of early Arctic coastal lifeways. Our research yielded direct dates of 4200 years ago for the oldest occupation of the site complex and identified several new sites dating to between 4200 and 2000 years ago. Results support the existing settlement model, pointing to increased sedentism and local population only after 2000 years ago. New data, however, indicate local population was much higher than previously established and that coastal occupation was sustained over long periods of time despite considerable mid-to-late Holocene paleoenvironmental variability. Together, these findings raise new questions about the evolution of maritime adaptations at high latitudes.
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