Background: rRNAs are produced as precursors and require nucleases for maturation. Results: Recombinant Rcl1 cleaves pre-rRNA at the in vivo A 2 site, and mutations abolish cleavage in vivo and in vitro. Conclusion: Rcl1 is the nuclease for co-transcriptional separation of rRNAs for the large and small subunit. Significance: Identification of the nucleases for rRNA production will allow accumulation and study of novel intermediates.

Using an assessment battery of psychological tests and clinical interview, 139 aspirants to the Permanent Diaconate in the Roman Catholic Church were evaluated as part of a screening process to determine who would be selected for a training program leading to ordination. This retrospective study was conducted to develop a systematic psychological description of those candidates deemed acceptable for training. Review of archival records identified differences between those aspirants accepted for training versus those not accepted on 13 key dimensions: MMPI-2 Factor 1-Anxious Distress; MMPI-2 Factor 2-Addiction/Acting Out; three scales of the Millon Index of Personality Styles (MIPS), Pain-Avoiding, Self-Indulging and Clinical Adjustment Index; marital satisfaction; education level; occupational stability; occupational satisfaction; service motivation; handling a hypothetical parish dilemma; DSM-IV-TR Axis I diagnosis and Axis II disorder/traits. Implications of how such a descriptive profile could be applied in future screening and selection of clergy candidates are discussed.

Introduction:We investigated the feasibility of incorporating the use of the personal digital assistant (PDA) in diabetes self-care in primary care.Methods: Adults with type 2 diabetes whose last measured HbA1c value was 8.0% or greater were recruited from 4 family practice clinics. A trained research assistant provided one-on-one training on the use of a loaned PDA preinstalled with Diabetes Pilot software.Results: Of 550 potential subjects invited for participation, only 98 (17.8%) called to schedule an orientation visit. However, 18 were never contacted when the recruitment goal was reached. Of the remaining 80 respondents, 43 (53.8%) met all study inclusion criteria. Participants' mean age was 55.2 years (SD ‫؍‬ 10.1). The majority were female (62.8%) and white (62.8%), 83.7% had at least some college education, and most reported an income of $30,000 to $69,999. The mean baseline HbA1c was 10.0% (SD ‫؍‬ 1.5). Major challenges of concern to the practicing family physician included few subjects agreeing to participate even though it was free, subjects who agreed to participate being generally different from those who decided not to participate, some PDAs not returned, and the relatively high cost of the intervention.Conclusions: Attempts to incorporate PDA use in diabetes self-care may be significantly challenging, although feasible. We identified several challenges and suggest strategies to overcome them.

Although medication discrepancies are common in elderly ambulatory patients, their family physicians appreciate assistance in correcting these discrepancies, although potential problems, such as cultural or organizational resistance to the open disclosure of medication discrepancies in medical records due to associated legal ramifications, may need to be resolved.

Cluster analysis was performed on a diverse group of 69 non-clinical grievers whose loved ones died between 12-40 months prior to the study. Based on psychometric measures of both bereavement distress and growth, three distinct clusters emerged: High Grief (high distress-low growth; n = 16); High Growth (low distress-high growth; n = 32); and Low Impact (low distress-low growth; n = 21). Discriminant function analyses showed that the High Grief cluster differed robustly from the other two groups on several measures of grief adaptation and coping; the differences between the High Growth and Low Impact clusters were less pronounced. High Grief participants sought grief counseling significantly more often whereas the Low Impact grievers preferred medicinal help for symptoms if they sought any professional assistance at all. When High Growth participants sought counseling, they seemed to focus on growth-oriented dimensions rather than mere symptom relief. Of the 11 participants who sought grief counseling for their loss, 10 (91%) found the experience to be helpful. Findings are discussed in the context of meta-analytic studies of the effectiveness of grief counseling.