Plant extracts have been proposed as substitutes for chemical feed additives due to their potential as rumen fermentation modifiers and because of their antimicrobial and antioxidant activities, possibly reducing methane emissions. This study aimed to evaluate the use of oregano (OR), green tea extracts (GT), and their association as feed additives on the performance and methane emissions from dairy between 28 and 87 d of lactation. Thirty-two lactating dairy cows, blocked into 2 genetic groups: 16 Holstein cows and 16 crossbred Holstein-Gir, with 522.6 ± 58.3 kg of body weight, 57.2 ± 20.9 d in lactation, producing 27.5 ± 5.0 kg/cow of milk and with 3.1 ± 1.8 lactations were evaluated (means ± standard error of the means). Cows were allocated into 4 treatments: control (CON), without plant extracts in the diet; oregano extract (OR), with the addition of 0.056% of oregano extract in the dry matter (DM) of the diet; green tea (GT), with the addition of 0.028% of green tea extract in the DM of the diet; and mixture, with the addition of 0.056% oregano extract and 0.028% green tea extract in the DM of the diet. The forage-to-concentrate ratio was 60:40. Forage was composed of corn silage (94%) and Tifton hay (6%); concentrate was based on ground corn and soybean meal. Plant extracts were supplied as powder, which was previously added and homogenized into 1 kg of concentrate in natural matter, top-dressed onto the total mixed diet. No treatment by day interaction was observed for any of the evaluated variables, but some block by treatment interactions were significant. In Holstein cows, the mixture treatment decreased gross energy and tended to decrease the total-tract apparent digestibility coefficient for crude protein and total digestible nutrients when compared with OR. During the gas measurement period, GT and OR increased the digestible fraction of the ingested DM and decreased CH expressed in grams per kilogram of digestible DMI compared with CON. The use of extracts did not change rumen pH, total volatile fatty acid concentration, milk yield, or most milk traits. Compared with CON, oregano addition decreased fat concentration in milk. The use of plant extracts altered some milk fatty acids but did not change milk fatty acids grouped according to chain length (short or long), saturation (unsaturated or saturated), total conjugated linoleic acids, and n-3 and n-6 contents. Green tea and oregano fed separately reduced gas emission in cows during the first third of lactation and have potential to be used as feed additives for dairy cows.

-The objective of this research was to determine the variation of milk stability evaluated with ethanol, boiling, and coagulation time tests (CTT) to identify milk components related with stability and verify the correlation between the three methods. Bulk raw milk was collected monthly at 50 dairy farms from January 2007 to October 2009 and physicochemical attributes, somatic cell (SCC), and total bacterial counts (TBC) were determined. Milk samples were classified into low, medium, and high stability to ethanol test when coagulation occurred at 72 °GL, between 74 and 78 °GL, and above 78 °GL, respectively. Univariate analysis was performed considering the effects of year, months, and interaction in a completely randomized design. Principal factor analysis and logistic regression were done. There was an interaction between months and years for stability to the ethanol test and coagulation time. All samples were stable at the boiling test. Boiling test was not related to ethanol and coagulation time tests. Coagulation time was weakly but positively correlated with ethanol test. Broken line analysis revealed that milk stability measured with CTT and ethanol tests decreased sharply when SCC attained 790,000 or 106 cell/mL of milk, respectively. Milk stability measured with ethanol test decreased when TBC was higher than 250,000 cfu/mL, while there was no inflexion point between TBC and stability measured with CTT. Milk with high stability presented lower values for acidity, TBC, and SCC but higher values for pH, lactose, protein, and CTT compared with lowstability milk. Due to the execution easiness, single-point cut-off result and low cost, we do not recommend the replacement of ethanol test for boiling or coagulation time test.Key Words: milk composition, seasonality, somatic cell count Revista Brasileira de Zootecnia

ABSTRACT. The experiment aimed at evaluating leaf biomass flow, intensity and frequency of defoliation of Alexandergrass (Urochloa plantaginea (Link) Hitch) by beef heifers exclusively on pasture or that grazed and received protein salt. A completely randomized experimental design was used with repeated measures over time, two treatments and two area replications, with three Angus test heifers by paddock. Leaf blade intake flow is 18% superior for heifers exclusively on pasture. Grazing intensity (59.8%) is similar when heifers receive protein salt or not. Heifers receiving protein salt reduce by one day the defoliation frequency of tillers. The supply of protein salt for beef heifers grazing Alexandergrass affects the herbivore-plant relationship, decreasing intake flow and leaf blade defoliation frequency.Keywords: defoliation frequency, defoliation intensity, morphogenesis, Urochloa plantaginea.Fluxos de biomassa e padrões de desfolhação em papuã pastejado por bezerras recebendo ou não sal proteinado RESUMO. O experimento foi conduzido para avaliar os fluxos de biomassa foliar, intensidade e frequência de desfolhação em papuã (Urochloa plantaginea (Link) Hitch), pastejado por bezerras de corte exclusivamente em pastejo ou em pastejo e recebendo sal proteinado. O delineamento foi inteiramente casualizado, com medidas repetidas no tempo, dois tratamentos e duas repetições de área, com três bezerras testes da raça Angus por piquete. O fluxo de consumo de lâminas foliares é 18% superior quando as bezerras permanecem exclusivamente em pastejo. A intensidade de pastejo (59,8%) é semelhante quando as bezerras recebem sal proteinado ou não. Bezerras que recebem suplemento reduzem em um dia a frequência de desfolhação dos perfilhos. O fornecimento de sal proteinado para bezerras de corte em pastejo em papuã resulta em alterações na relação herbívoro-planta reduzindo o fluxo de consumo e a frequência de desfolhação de lâminas foliares.Palavras-chave: frequência de desfolhação, intensidade de desfolhação, morfogênese, Urochloa plantagine.

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