A new coupled cloud point extraction (CPE) was developed as prompt, easy and economical preconcentration technique for spectrophotometric determination of tiny amounts of Fe 3+ and Hg 2+ in real samples. A modern thiazolylazo ligand [methyl phenyl thiazolyl azo]-3-methyl-4-methoxy-2-naphthol (MPTAN) was done for the CPE method in the preconcentration of Fe 3+ and Hg 2+ as an earlier step to its characterization by UV-VIS spectrophotometry. The analytical manner includes the formation of under study metals complex with new ligand and quantitatively extracted to the Cloud point layer (CPL) of TritonX-100 after heating. The concentration of MPTAN, metals, pH, thermodynamic data and volume of surfactant was optimized. The investigation of stoichiometry has the ratio of metal to ligand of 1:1. Under finest settings and conditions, the calibration curve was found to be linear in the concentration range of two ions (0.05-10) ppm and the limit of detection for Fe 3+ and Hg 2+ are (0.016 and 0.041) ppm respectively. The suggested CPE has an excellent methodology for characterization of trace metal ions in surrounding samples with a complex matrix as in soils, water, vegetable, meats, and fruits.

By joined two sensitive methods which in onium method and Cloud point extraction method CPE for separation preconcentration and extraction Fe 3+ and Hg 2+ by using (BAEE) from acidic hydrochloric aqueous solution in the presence TritonX-100. Spectrophotometric studies shows wave length for maximum absorbance to onium species of Fe(III) was λ max =362nm and for Hg(II) λ max =293nm. Optimum condition studies was 1M HCl for both ions, 100µg of each metal ions ,using 1×10 -4 M of (BAEE), as well as the method need heating for 85 0 C to Fe 3+ and 95 0 C for Hg 2+ ion and heating time 15min. for Fe 3+ and 20min. for Hg 2+ ion. so that need existance 0.5mL of TritonX-100 for both metal ion, the research involved many other studies. keyword: Cloud point extraction, onium system, Na-Benzoyl-L-arginine ethyl ester hydrochloride (BAEE), mercury, iron.

The cloud point extraction technique has become increasingly popular in recent years for trace metal separation and preconcentration. When heated to a specific temperature, cloud point extraction utilizes the property of nonionic surfactants in aqueous solutions to generate micelles and become turbid (so-called cloud point temperature). For analytical chemists, developing a simple and selective technology for the separation and determination of metals and medicinal drugs is a critical concern. Therefore, a sensitive, accurate, and green cloud point extraction (CPE) procedure was developed for the micro-determination of metal cations like zinc (II) and cadmium (II) in food samples. Triton X–114 and 1-(4-(Phenyldiazenyl) phenyl) azo naphthalene-2-ol (Sudan III) were used as extractants. Sudan III forms an ion-pair association complex with metal ions when the pH is 9. In the presence of 5 ppm zinc (II) or 4 ppm cadmium (II) in an aqueous solution, the maximum extraction efficiency should be achieved. In order to create Cloud Point Temperature (CPT) quantitatively, the extraction applications in this approach required heating at 85°C for 20 minutes. In this work, the impacts of different surfactants, pH, stoichiometry, and various organic reagents on interferences as well as spectrophotometric determination were explored. The linearity ranges of zinc (II) and cadmium (II) were 0.25-700 and 0.25-400 ppm, respectively. The results show low detection limits of 0.035 and 0.042 ppm for zinc (II) and cadmium (II), respectively. Also, the quantification limits for zinc (II) and cadmium (II) are 0.116 and 0.140 ppm, respectively.

Extracted Mg(II) and Ca(II) each one alone after combined with EDTA (H 3 Y -) as (MgHY -) and (CaHY -) after preparing ion pair complexes according to liquid ion exchange method by using Cinchonine (CK) after changing to liquid ion exchanger HCK + ;Cl -in HCl medium, spectrophotometric studies showed that the ion pair magnesium complex HCK + ;MgHY -had a wave length for maximum absorbance of λ max =241nm, but the maximum absorbance for calcium HCK + ;CaHY -was λ max =278nm to determine the remaining quantity of Mg(II), the Eriochrome black T method was used, but for Ca(II) the crown ether DB18C6 method was used as new spectrophotometric method. Limitations to the study include optimum conditions for extraction ions in this study show a need for 1M HCl for Mg(II) and 0.8M HCl for Ca(II), to be present 100µg for each ion, 0.08M EDTA for Mg(II) and 1.0M EDTA for Ca(II), 1×10 -4 M CK, after shaking for 10 min.

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