To clone zinc finger genes expressed in hematopoietic system, we designed primers based on conserved Cys 2 / His 2 zinc finger sequences to amplify corresponding domains from mRNA of normal bone marrow and leukemia cell line NB4. DNA fragments of novel zinc finger genes were chosen and used as probe pool to screen cDNA libraries or subject to rapid amplification of cDNA ends in order to obtain full-length cDNA. Transcriptional regulation is controlled through interaction between DNA and protein complex, the latter containing transcription factors with highly conserved protein motifs. The most well known motifs are the helix-turn-helix, helix-loophelix, and zinc finger. During cell differentiation and development, each of these domains is involved in the binding of transcription factors to their cognate DNA recognition site, resulting in the specific activation or repression of gene expression (1).
LOH at the p53 gene locus is a frequent event in multiple step carcinogenesis progression. The high frequency of LOH at 17p13.3 suggests that there may be another tumor suppresser gene in that chromosome region.
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