The development of biosensors capable to achieve accurate and precise molecular measurements in the living body under pH-variable biological environments (e. g. subcellular organelles, biological fluids and organs) plays a...
The electrochemical aptamer‐based (E‐AB) biosensor usually has a long reaction time when detecting thrombin. This work reports the design of an E‐AB biosensor with dual recognition sites to quickly detect thrombin. Specifically, two specific recognition sites of thrombin were used to design three aptamer sequences (TBA‐15, TBA‐29 and TBA‐U), followed by fabrication of corresponding sensors. First, we tested these three types of biosensors in tris buffer solution, and found that the response time of the TBA‐U sensor to the same concentration of thrombin was about 2 hours, which is shorter than TBA‐15 and TBA‐29 sensors. Then, we also did the same test in 50 % diluted serum with 500 nM thrombin. The response time of the TBA‐U sensor was about 2 hours, which is still faster than the 3 hours of TBA‐15 sensor and the 5.5 hours for TBA‐29 sensor. In addition, in terms of dynamic range and specificity, TBA‐U has good performance.
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