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aThe graphene oxide (GO) nanosheets modified cement tremendously improved their mechanical properties but decreased the fluidity. The adsorption characteristics between GO and cement plays a pivotal role for the influence on fluidity. In this paper, the adsorption characteristics of graphene oxide nanosheets on cement were investigated by scanning electron microscope (SEM), total organic carbon (TOC), Fourier transform infrared spectra (FTIR) and XPS. The results show that GO nanosheets have a strong adsorption ability on the cement surface. And the adsorption data was best fitted with the Freundlich adsorption isotherm model and described by the pseudo-second order kinetics model, the adsorption process contained the chemical reaction that the -COOH on the edge of GO nanosheets reacted with the metal cations. The adsorption layer thickness of GO nanosheets on cement was about 10.16 nm.