The aim of the study was to analyse the smear layer removal at the coronal,middle and apical third of the root canals by using Er, Cr; YSGG laser system equipped with conical tip of 300 µm. A total of 80 single rooted extracted human mandibular premolars were endodontically prepared with rotary instrumentation and irrigated with 5.25% sodium hypochlorite and were randomly divided in to four groups (20 tooth each). GROUP I: Served as control (non lased group), in which root canal were irrigated with 17% EDTA .GROUP II : 17% EDTA irrigant activated by laser with conical fibre tip at the coronal region .GROUP III: 17% EDTA irrigant activated by laser with conical fibre tip at the middle region.GROUP IV: 17% EDTA irrigant activated by laser with conical fibre tip at the apex region.The results showed complete smear layer removal at the coronal and middle region when compared to apical third of the root canal system.
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