Introduction: The voltammetric sensing of Acetaminophen (AN) using modified Dysprosium Copper Oxide (DyCuO) Nanoparticles (NP) mixed Carbon Paste Electrode (MCPE) was successfully developed. Methods: The modification of bare NPMCPE was achieved by the polymerisation of DL-Phenylalanine (DLPA). The electroanalysis of the AN was achieved by utilizing the Cyclic voltammetry (CV) approaches. The crystallographic nature of the nanoparticle was studied via X-ray Powder Diffraction (XRD) technique. The surface morphology and electrochemical feature of the prepared electrode were evaluated by Field Emission Scanning Electron Microscopy (FE-SEM) and Electrochemical Impedance Spectroscopy (EIS) techniques. Results: The modified sensor exhibited an excellent electrocatalytic activity towards the electroanalysis of the AN. Several aspects, such as the number of polymerisation cycles, variation of pH, and the impact of scan rate were investigated in 0.2 M supporting electrolyte (pH 7) at a sweep rate of 0.1 Vs-1. The suggested sensor shows a very low detection limit (11.95×10-8 M) with a linear range of 2.0 to 50.0 µM, which exhibits excellent sensitivity. Conclusion: The stable and reusable sensor was applied for the estimation of AN in the tablet sample. Thus, P(DLPA)MNPMCPE was utilized as the most capable sensor for the voltammetric detection of AN.
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