Following the discovery of the semi-synthetic anticoccidial ionophore semduramicin1'2* (UK-61,689), a mutation program was undertaken to produce this polyether antibiotic by direct fermenta-tion3). The producing culture of the antibiotic UK-58,852, Actinomadura roseorufa ATCC53666, was chosen as the parental strain to be mutagenically treated to induce a semduramicin-producing mutant4'5). The two compounds differ from each other in that the A-ring sugar moiety of UK-58,852 is replaced by a hydroxyl group in semduramicin. Recently, we described the formation of two new polyether antibiotics, CP-91,243 and CP-91,244,
Anew monocarboxylic acid ionophore antibiotic related to zincophorin, CP-78,545 (1). was found in the culture broth of Streptomyces sp. N731-45. CP-78,545 was extracted with organic solvents and purified by column chromatography. The metabolite, which is active in vitro against certain Gram-positive bacteria, as well as the anaerobe Treponema hyodysenteriae, and a coccidium Eimeria tenella, was isolated as a water insoluble magnesiumsalt (2) in 2 : 1 (ligand/metal) stoichiometry. The structure of CP-78,545 was elucidated by spectroscopic (NMRand MS) methods, and the relative stereochemistry was determined by single-crystal X-ray analysis of the cadmium salt (3). CP-78,545, i.e., 24-dehydrozincophorin, is unique since its molecular backbone contains a terminal double bond previously not found in other polyether ionophores.
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