Streptomyces
strains are powerhouses for a diverse range of secondary metabolites, including antibiotics, anticancer and immunosuppressive agents, and enzymes. Here, we report the genome sequence of
Streptomyces
sp. strain PSAA01, which was isolated from a soil sample taken in Manas National Park, Assam, India, in the eastern Himalayan foothills of India.
The main effectors in the innate immune system of Bombyx mori L. are antimicrobial peptides (AMPs). Here, we infected B. mori with varied inoculum sizes of Pseudomonas aeruginosa ATCC 25668 cells to investigate changes in morpho-anatomical responses, physiological processes and AMP production. Ultraviolet-visible spectra revealed a sharp change in λ max from 278 to 285 nm (bathochromic shift) in the hemolymph of infected B. mori incubated for 24 h. Further, Fourier Transform InfraRed studies on the hemolymph extracted from the infected B. mori showed a peak at 1550 cm −1 , indicating the presence of α-helical peptides. The peptide fraction was obtained through methanol, acetic acid and water mixture (90:1:9) extraction, followed by peptide purification using Reverse Phase High Performance Liquid Chromatography. The fraction exhibiting antibacterial properties was collected and characterized by Matrix-Assisted Laser Desorption/Ionization-Time of Flight. A linear α-helical peptide with flexible termini (LLKELWTKMKGAGKAVLGKIKGLL) was found, corresponding to a previously described peptide from ant venom and here denominated as Bm-ponericin-L1. The antibacterial activity of Bm-ponericin-L1 was determined against ESKAPE pathogens. Scanning electron microscopy confirmed the membrane disruption potential of Bm-ponericin-L1. Moreover,
Background One of the most widely consumed fruits in the world, is the banana (Musa sp.). Endophytic bacteria found in bananas have the ability to stop the growth of pathogenic fungi like leaf spot disease.Methods and Results The goal of the research was to identify and examine two endophytic bacteria's potential as antifungal agents against the leaf spot disease and other plant growth promoting (PGP) activities. The steps in this study are sample collection from the soil, the roots, and diseased leaves, isolation, antagonist assays of two endophytic bacteria (MRH − 06 and MRH − 11) against the fungus pathogen (MLP-01), and identification using 16S rDNA and 18s rDNA partial sequencing. Enterobacter cloacae (ON955844) and Achromobacter xylosoxidans (ON955872) the two bacterial isolates showed antagonistic activity against the fungal pathogen Curvularia lunata (ON246070).Conclusion Utilizing such potent endophytes to restrict or suppress phytopathogenic activity is an alternative method and these have great potential in agricultural in future.
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