Copper nanoparticles (Cu NPs) have been synthesized by using laser ablation method, using deionized water as main solvent. The formation of Cu NPs is confirmed by UV-visible spectrophotometer (UV-Vis), atomic force microscopy (AFM) and X-ray diffraction (XRD). Cu NPs fabricated by laser ablation have diameter in the range from 14 to 55 nm. Structural analysis revealed the face-centered cubic (fcc) crystal structure of Cu NPs. The antibacterial activity of Cu NPs has been evaluated in vitro against strains of Escherichia coli (E. coli) and Staphylococcus aureus (S. aureus). The fabricated Cu NPs show considerable antibacterial activity against both bacterial strains. The bacterial activity of Cu NPs was found to depend on the microbial species.
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