Background. Work-related upper extremity musculoskeletal disorders (WUEDs) often present remarkable health and economic burdens on society. Occupational barbers are usually neglected both in research and policy actions, mainly in developing countries, and hence are likely subjected to the conditions. So far, information about factors that influence WUEDs among barbers in Ethiopia is inconclusive. Therefore, this study aimed to evaluate prevalence and factors associated with WUEDs among barbers in Gondar town, Ethiopia. Methods. We conducted a cross-sectional study from March to April 2018. A sample of 424 participants were recruited using systematic random sampling technique. A standardized Nordic Musculoskeletal Questionnaire was pretested and interviewer-administered for data collection. The data were analyzed by SPSS version 20 software. We set statistical significances at <0.05 p value with 95% confidence intervals (CI) and computed odds ratios to evaluate strength of associations. Results. The response rate was 98.3% (N=417). The mean age was 26.39 (SD + 4.805) years. The prevalence of upper extremity musculoskeletal disorders over the past 12 months was 56.8% (N=237). Upper back pain was observed in 38.8% (N=162) participants, whereas shoulder (27.1% (N=113)), neck pain and elbow/forearms (each 29.3% (N=122)), and wrists/hand disorders (32.4% (N=135)) were the common body sites indicated. Age (AOR: 2.614; 95% CI (1.287, 5.307)), alcohol use (AOR: 3.556; 95% CI (2.212, 5.717)), frequent standing (AOR: 1.536; 95% CI (1.006, 2.346)), physical exercises (AOR: 1.938; 95% CI (1.216, 3.089)), and low monthly salary (AOR: 3.125; 95% CI (1.157, 5.441)) were factors associated with work-related upper extremity disorders. Conclusions. Work-related upper extremity disorder is common among hairdressing professionals. Worksite health promotions targeted to lifestyle behaviors, like physical exercise and alcohol consumption require urgent public health actions in Ethiopia. Moreover, adaption of flexible work postures and proper management of workplace conditions related to aging workforces are also imperative to trace the complaints.