Introduction: Viruses are responsible for two-thirds of all acute respiratory tract infections. This study aims to retrospectively detect respiratory tract viruses in patients from all age groups who visited the hospital.
Methodology: A total of 1592 samples from 1416 patients with respiratory tract symptoms were sent from several clinics to the Molecular Microbiology Laboratory at Gazi University Hospital from February 2016 to January 2019. Nucleic acid extraction from nasopharyngeal swabs, throat swabs or bronchoalveolar lavage (BAL) samples sent to our laboratory was done using a commercial automated system. Extracted nucleic acids were amplified by a commercial multiplex-real time Polymerase Chain Reaction (PCR) method, which can detect 18 viral respiratory pathogens.
Results: Among 1592 samples, 914 (57.4%) were positive for respiratory viruses. The most prevalent were rhinovirus (25.2%) and influenza A virus (12.1%), the least prevalent was the bocavirus (2.6%). Rhinovirus was the most detected as a single agent (21.2%, 194/914) among all positive cases, followed by coronavirus (9.3%, 85/914). The detection rates of coronavirus, human adenovirus, respiratory syncytial virus A/B, human parainfluenza viruses, human metapneumovirus-A/B, human parechovirus, enterovirus and influenza B virus were 9.9%, 8%, 7.7%, 5%, 3.4%, 3.1%, 3%, and 2.8%, respectively.
Conclusions: The most detected viral agents in our study were influenza A virus and rhinovirus. Laboratory diagnosis of respiratory viruses is helpful to prevent unnecessary antibiotic use and is essential in routine diagnostics for antiviral treatment. Multiplex Real-time PCR method is fast and useful for the diagnosis of viral respiratory infections.
Yeni ortaya çıkan ve yeniden önem kazanan bulaşıcı hastalıklar dünya genelinde artmaktadır. Son otuz yılda Ebola, Nipah, Hendra, Zika, şiddetli akut solunum yolu sendromu (SARS), Ortadoğu solunum sendromu (MERS) ve yeni (Novel) koronavirüsler (COVID-19) dâhil olmak üzere 30'dan fazla yeni insan patojeni keşfedilmiştir ve bunların %75'inin hayvanlardan kaynaklandığı bilinmektedir. Virüsler, insanlarda yaşamsal tehlike oluşturan, birçok hastalığa neden olan ve insan evrimi ile birlikte gelişen, etkili bulaşıcı ajanlardır. Yeni ortaya çıkan özellikle RNA virüsleri, yeni bir konakçıya ve değişen ortamlara diğer patojenlerden daha hızlı bir şekilde adapte olma potansiyeline sahiptir. Son yıllarda artış gösteren konak, çevre ve mikroorganizmalarla ilgili birçok faktör yeni ortaya çıkan ve yeniden önem kazanan enfeksiyon hastalıklarını gündeme getirmektedir.
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