A new, simple and sensitive spectrophotometric method is developed for the determination of ranitidine hydrochloride (RNH). The proposed method is based upon reaction of RNH with 9-fluorenylmethyl chloroformate (FMOC-Cl) in borate buffer of pH 8.0 producing an absorption maximum at 255 nm. All parameters required for the reaction conditions are investigated. Linearity is verified with a range of 2-16 μg/mL and is described by the regression equation y = 61129 x + 0.0354 with a correlation coefficient of 0.9998 (n = 7). The limit of detection (LOD) and the limit of quantification (LOQ) were calculated as per ICH guidelines and were found to be 0.2219 and 0.6724 μg/mL, respectively. The method was successfully applied for the determination of RNH in pharmaceutical formulation. Therefore, the method can be used for routine analysis of RNH in quality control laboratories.
A new, simple, precise, accurate and selective method for ranitidine hydrochloride (RNH) quantification was developed based on the electrochemical oxidation of RNH on the pencil graphite electrode (PGE) modified with p-amino benzene sulfonic acid/cucurbit( 6) uril (p-ABSA/CB ( 6)). During analysis, an electrochemical cell was employed with a conventional three-electrode system and (0.1molL -1 ) potassium chloride KCl solution as the supporting electrolyte. Under optimal experimental conditions, the peak current response increases linearly with a ranitidine concentration over the range of 2×10 -4 -1.7×10 -2 molL -1 . The detection limit of the method was estimated to be 1.5746×10 -4 molL -1 . To further validate its possible application, the proposed method was successfully used for the determination of RNH in pharmaceutical formulation. The scanning electron microscope was accomplished to investigate the structural behavior of the film that originated on the electrode surface.
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