This study was aimed to detecting three different virulence genes (UreC, HmpA and ZapA ) for Proteus mirabilis isolated from different clinical samples ( urine from UTI non-Rheumatoid arthritis patients, urine from UTI Rheumatoid arthritis patients, wound swaps , chronic supportive otitis media(CSOM) and cerebrospinal fluid aspirate(CSF). Three hundred (300) different clinical samples were collected and cultured on blood agar and macConky agar. 40 of samples were identified as P. mirabilis , biochemical test and VITEK 2 system used to complete the identification. Phenotypic detection appeared the present 100% of urease enzyme (on urea agar) hemolysin enzyme ( on blood agar), swarming phenomena (on agar plate ) while protease enzyme detection (on skim milk agar) present in 54% of isolates. Molecular results by PCR present UreC, HmpA and ZapA genes in 100 % in 40 isolates. Antibiotic Susceptibility test of Proteus mirabilis based on Kirby-Bauer disk diffusion method on Muller-Hinton agar to (14) different antimicrobial agents appeared 100% resistance to Aminoxicillin /clav, Cefotaxime and Erythromycin while the lowest resistance was against Impenem, Ciprofloxacin, Meropenem and Gentamicin (12.5%, 15 %, 20% and 47.5%), respectively.
This study was aimed to detecting difference in proteins separation by SDS-Page for Proteus mirabilis isolated from urine of non-Rheumatoid arthritis patients have urinary tract infection , urine from Rheumatoid arthritis patients have urinary tract infection. Two hundred (200) urine sample collected from non RA patient30 urine sample from RA patient . These clinical samples were collected and cultured on blood agar and macConky agar. 32 isolates were identified as p. mirabilis by using biochemical test and VITEK 2 system used to complete the identification. The current study used SDS-Page technique for proteins analysis of Proteus mirabilis for both RA patient and non RA patient to detect the differences between them and the result appeared that there are no differences in total protein, insoluble proteins and soluble protein at different pH. Values (6.0, 7.0 & 8.0) at 37 cº, also the result shown differences in the cell –free culture supernatants between Proteus mirabilis from urine of both rheumatoid arthritis patients and non-rheumatoid arthritis patient in pH.8.0 at 37 cº in 7 h. by band appeared in pH.8.0 at 37 cº in 7 h in RA .and there is no band in non RA at the same condition.
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