α-Zein
has received widespread attention owing to its unique
solubility, amphipathic, and self-assembly properties, which is because
of its high proportion of nonpolar amino acids and unique amino acid
sequence. The protein self-assembly is a significant and widely observed
phenomenon in many scientific areas such as food and biomedicine,
among many industries. In this study, we investigated the self-assembly
behavior of α-zein and regulated the morphology and structure
of the self-assembled α-zein by varying the experimental parameters
like pH, ethanol content, induction time, and α-zein concentration
during the self-assembly process in ethanol–water mixtures.
The nanospheres and nanofibers were observed under different conditions
[nanospheres observed under acidic and strongly alkaline (pH >
10.5)
conditions or for ethanol content lower than 65% and higher than 75%;
nanofibers observed under weakly alkaline (pH 9.5–10.5) conditions
or for 65–75% ethanol concentration for induction duration
longer than 24 h]. The morphological and structural analyses of the
self-assembled α-zein showed that the self-assembly process
was accompanied by the transformation of the morphology and conformation
of α-zein. The studies on the self-assembly process and mechanism
revealed that α-zein first self-assembled into nanospheres,
followed by the nanospheres adhering to shape-beaded fibers and finally
fibers, accompanied by a structural transformation from the disordered
into ordered state. The nanosphere formation is noted to follow the
nucleation-based polymerization, and the nanosphere-mediated mechanisms
lead to the formation of nanofibers. Moreover, the hydrophobic interactions,
hydrogen bonds, and electrostatic interactions are concluded to drive
the α-zein self-assembly. The findings from this study are expected
to provide a theoretical basis for expanding the commercial applications
of α-zein.
Polyvinyl alcohol (PVA) and benzoguanamine (BG) modified melamine-formaldehyde (MF) resins were used to prepare high-pressure laminates (HPLs) and the improved tensile strength, flame retardancy and antifouling properties were investigated.
Oil released into the environment becomes one of the foremost concerns that rigorously impact the environmental system. Therefore, there is needed to develop newer membranes, modify existing membranes, or tune...
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