The scaling relationship between leaf dry mass and leaf surface area has important implications for understanding the ability of plants to harvest sunlight and grow. Whether and how the scaling relationships vary across environmental gradients are poorly understood. We analyzed the scaling relationships between leaf mass and leaf area of 121 vascular plant species along an altitudinal gradient in a subtropical monsoon forest. The slopes increased significantly with altitude, it varied from less than 1 at low altitude to more than 1 at high altitude. This means that plants growing at high altitude allocate proportionately more biomass to support tissues in larger leaves and less in smaller leaves, whereas the reverse is true at low altitude. This pattern can be explained by different leaf strategies in response to environmental pressure and constrains.

Reactive oxygen species (ROS) have been established to participate in stomatal closure induced by live microbes and microbe-associated molecular patterns (MAMPs). Chlorella as a beneficial microorganism can be expected to trigger stomatal closure via ROS production. Here, we reported that Chlorella induced stomatal closure in a dose-and time-dependent manner in epidermal peels of Vicia faba. Using pharmacological methods in this work, we found that the Chlorella-induced stomatal closure was almost completely abolished by a hydrogen peroxide (H2O2) scavenger, catalase (CAT), significantly suppressed by an NADPH oxidase inhibitor, diphenylene iodonium chloride (DPI), and slightly affected by a peroxidase inhibitor, salicylhydroxamic acid (SHAM), suggesting that ROS production involved in Chlorella-induced stomatal closure is mainly mediated by DPI-sensitive NADPH oxidase. Additionally, Exogenous application of optimal concentrations of Chlorella suspension improved instantaneous water use efficiency (WUEi) in Vicia faba via a reduction in leaf transpiration rate (E) without a parallel reduction in net photosynthetic rate (Pn) assessed by gas-exchange measurements. The chlorophyll fluorescence and content analysis further demonstrated that short-term use of Chlorella did not influence plant photosynthetic reactions center. These results preliminarily reveal that Chlorella can trigger stomatal closure via NADPH oxidase-dependent ROS production in epidermal strips and improve WUEi in leave levels.

Sugars act as vital signaling molecules that regulate plant growth, development and stress responses. However, the effects of sugars on stomatal movement have been unclear. In our study, we explored the effects of monosaccharides such as glucose and mannose on stomatal aperture. Here, we demonstrate that glucose and mannose trigger stomatal closure in a dose- and time-dependent manner in epidermal peels of broad bean (Vicia faba). Pharmacological studies revealed that glucose- and mannose-induced stomatal closure was almost completely inhibited by two reactive oxygen species (ROS) scavengers, catalase (CAT) and reduced glutathione (GSH), was significantly abolished by an NADPH oxidase inhibitor, diphenylene iodonium chloride (DPI), whereas they were hardly affected by a peroxidase inhibitor, salicylhydroxamic acid (SHAM). Furthermore, glucose- and mannose-induced stomatal closure was strongly inhibited by a Ca(2+) channel blocker, LaCl3 , a Ca(2+) chelator, ethyleneglycol-bis(beta-aminoethylether)-N,N'-tetraacetic acid (EGTA) and two water channel blockers, HgCl2 and dimethyl sulfoxide (DMSO); whereas the inhibitory effects of the water channel blockers were essentially abolished by the reversing agent β-mercaptoethanol (β-ME). These results suggest that ROS production mainly via NADPH oxidases, Ca(2+) and water channels are involved in glucose- and mannose-induced stomatal closure.

The changes in plant–plant interactions along environmental gradients have been a focus of recent ecological research. It has been suggested that both above‐ and below‐ground competition and their interplay vary along gradients, but few studies have investigated this idea, and in most cases, the role of facilitation has not been considered, despite its importance in high stress environments. Here we used two‐layer ‘zone‐of‐influence’ models to simulate the effects of facilitation, size‐asymmetry of competition, abiotic stress, resource availability and the balance of root–shoot growth on shoot and root interactions and their interplay along an environmental gradient. In the absence of facilitation, shoot and total competition became weaker, while root competition and the interplay between shoot and root competition were unchanged under increasing stress when root competition was completely symmetric. In contrast, shoot, root, total interactions and the interplay between shoot and root interactions were all negative, and they increased with increasing stress when root competition was size‐symmetric. When facilitation was included in the models, net effects of shoot, root, total interactions and the interplay of root–shoot interactions were very different from those without facilitation, and many were positive under highly stressful conditions. The type of stress (non‐resource or resource) did not significantly influence the simulation results. Our study provides an alternative interpretation of the interplay between above‐ and below‐ground plant–plant interactions across an environmental gradient.

Microbes-and microbe-associated molecular patterns-induced stomatal closure have been known to be connected with the early defense responses and the improvement of instantaneous water use efficiency (WUE i ) in plants. Being a commercially available microorganism, Bacillus subtilis can promote plant growth and induce disease resistance. However, its effects on stomatal movement and WUE i in plants have been largely unexplored. Here, we showed that B. subtilis induced stomatal closure in a dose-and time-dependent manner when applied to isolated epidermal peels and intact leaves of broad bean. Pharmacological study further revealed that the B. subtilis-induced stomatal closure in epidermal peels was mediated mainly by reactive oxygen species production via NADPH oxidases. Furthermore, foliar application of B. subtilis significantly reduced stomatal aperture, stomatal conductance (g s ), transpiration rate (E) and net photosynthesis rate (P n ) of leaves of broad bean at 8 and 24 h, reductions in which were reversed after 48 h. As a consequence, the WUE i of plants treated with B. subtilis for 8-144 h was higher than that in the control. The chlorophyll fluorescence and content analysis further demonstrated that B. subtilis could enhance plant photosynthetic activities by increasing leaf photosynthetic efficiency and chlorophyll content. These results suggest that foliar spray of B. subtilis can improve WUE i of crop plant via the regulations of stomatal movement and photosynthetic activity during a special time period.