Allergenic pollens are found in gardens, fields and green spaces around cities. In Kermanshah, located in the west of Iran, seven allergen pollen samples were collected directly from nature and studied according to the Erdtman acetolysis method by using Light Microscope (LM) and Scanning Electron Microscope (SEM). In this study, atmospheric pollens were also examined by the Durham method. The results showed that the pollen grains were small, medium, and large in terms of size, and Triporate, Pantoporate, Colpate and Tricolporate in terms of aperture. In addition, they were Striate, Perforate, Macroechinate, Microechinate, and Psilate with regard to exine ornamentation; however, the echinate type was more common. In general, Prolate to a spherical shape, mediumsized, perforate, and echinate pollen characteristics are popular in the important allergenic plants. Therefore, due to the human's manipulations in nature and also the extensive size of gardens and fields, there are many types of weeds in Kermanshah Province which are significant in terms of being allergenic.
In this research, the pollen micromorphology of nine species belonging to Honorius, Loncomelos, Ornithogalum and Stellarioides are observed by LM (Light Microscopy), SEM (Scanning Electron Microscopy) and two species by TEM (Transmission Electron Microscopy). Lately, the phylogenetic studies have determined nineteen monophyletic genera in subfamily Ornithogaloideae (=tribe Ornithogaleae). Our results show that the pollen grains characters such as shape, size, exine ornamentation on equatorial surface, sulcus margin exine ornamentation, sulcus membrane ornamentation and lumina number on exine surface are varied among all studied genera. The dendrogram of palynological characters analyzed on LM and SEM observation by MVSP (Multi Variate Statistical Package) software partly supports the monophyletic genera. According to the obtained palynological dendrogram, Loncomelos arcuatum and Ornithogalum sintenisii are placed in one subtype. Based on the sporoderm structure (exine thickness and the endexine type), L. arcuatum differs from O. sintenisii. Finally, the palynological characters can be useful for clarifying the taxonomic relationship in this subfamily.
Aims:The aim of this research was to investigate the morphological characteristics of the pollen grain of some species of genus Allium. Methodology: The pollen grains were examined by using Light Microscopy (LM), Scanning Electron Microscopy (SEM) and the pollen grains of one species under Transmission Electron Microscopy (TEM). Results: The pollen grains were oblate and medium in shape and size. The pollen ornamentation of exine surface, exine ornamentation on sulcus edge, lumina number in the exine surface and the state of pollen grain apex in the examined species were different. Semitectate and columellate ectexine with discontinuous endexine were seen in the pollen wall structure (sporoderm). The dendrogram obtained from the pollen characters in SEM observations by using the numerical taxonomy system (NTSYS) software placed the studied species in two types.
Original Research ArticleConclusion: Our palynological dendrogram can be used for segregation the sections and subgenera taxonomical levels in the studied species of genus Allium and confirmed the phylogram of the recent phylogenetic research.
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