Selection through molecular markers for seed Zn accumulation might be an
efficient complementary breeding tool in barley breeding. To develop a
specific molecular markers, 150 DH lines derived from a cross between Clipper
(low-Zn-accumulator) and Sahara-3771 (high-Zn-accumulator) were screened
under field and glasshouse conditions. Microsatellite-anchored fragment
length polymorphism (MFLP) fingerprint generated by SSR-anchor primer MF128
in combination with AFLP primer MseI-AGA (5?-GATGAGTCCTGAGTAAAGA-3?) was
identified as a candidate marker for tagging seed Zn accumulation gene. The
sequencing of the band showed a marker of 369 bp with the sequence of SSR
anchor primer MF128 and MseI-AGA at the two ends as expected. The MFLP marker
associated with higher seed Zn accumulation has potential to be converted to
a simple, sequence-specific, PCR-based, low-cost marker amenable to large
populations, making it potentially viable for marker-assisted selection in
barley breeding. This marker might be useful in the improvement of barley
productivity and nutritional quality in Zn-deficient environments.
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