The objective of the current study was to evaluate the antioxidant activity and enzyme inhibitory effect of different parts of medlar including fruit, leaf and flower bud by using various in vitro methods, and also determination of total phenolic and flavonoid content in the samples. Ethanol extracts of medlar parts were prepared and their antioxidant activities were determined using 1,1-diphenyl-2-picryl-hydrazil (DPPH•) scavenging and β-carotene bleaching methods. The leaf extract showed the strongest antioxidant activity. DPPHradical scavenging activity was in the order of BHA > leaf > bud > fruit. This ordering was the same for β-carotene bleaching activity, tocopherol > leaf > bud > fruit. The highest total phenolic (60.3 ± 1.69 mg GAE g-1 extract) and flavonoid (14.77 ± 1.15 mg QE g-1 extract) content were determined in leaf extract. For possible antidiabetic effects of extracts, α-amylase and α-glucosidase inhibitory activities were investigated, the bud extract showed the highest inhibition activities among the all extracts.
Klinik numunelerde yapılan kalitatif ve kantitatif tayinlerin hastaların sağlığı açısından kritik bir öneme sahip olduğu bilinmektedir. Klinik analizlerin gerçekleştirildiği cihazların performans ve kaliteleri, sonuçların doğruluğunu etkilemektedir. Hastalığın tanısının konması ve tedavide doz ayarının yapılabilmesi gibi çok önemli kararların alınabilmesi, cihazların performansına bağlıdır. Bu çalışmada klinik uygulamalar arasından toksikolojik analiz, steroid hormon ölçümü, tam kanda takrolimus seviyesi ve idrarda bisfenol A analizleri için ELİSA (enzim bağlı immunosorbent analizi) ve LC-MS (sıvı kromatografi-tandem kütle spektrometresi) tekniği karşılaştırılmış ve analiz seçimini nasıl etkilediği araştırılmıştır.
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