Effect of sulfuric acid (SA) on the heat treatment of electrospun polyacrylonitrile (PAN) fibers and their application on guanine oxidation detection in single‐strand DNA molecules (ssDNA) were studied. In this regard, two different approaches were performed. Aqueous sulfuric acid solutions were poured on the pure PAN nanofibers and heat treatment process was performed to determine the maximum temperature at which fiber structures does not deteriorate. Also, SA was directly added into electrospinning solutions with different ratios and heat treatment process was applied to the as‐spun PAN/SA fibers. The fibers were produced both directly on a grounded plate and cylindrical pencil graphite surfaces (PGE) for the fiber characterizations and biosensor measurements. Single strand DNA (ssDNA) molecules were immobilized on the samples for the electrochemical investigation of guanine oxidation signal. 0.971 and 3.69 μA guanine oxidation signal intensities were detected for neat PGE and heat‐treated PAN/SA fiber coated PGE. The formation of sulfonic groups on the fibers has driven more ssDNA attachment on the surface, and hence increased the guanine oxidation signal intensity dramatically (about 261.4% increment). The results have shown promising future of heat‐treated PAN/SA micro/nanofibers for sensitive detection of guanine oxidation signal from genetic molecules.
scite is a Brooklyn-based organization that helps researchers better discover and understand research articles through Smart Citations–citations that display the context of the citation and describe whether the article provides supporting or contrasting evidence. scite is used by students and researchers from around the world and is funded in part by the National Science Foundation and the National Institute on Drug Abuse of the National Institutes of Health.