The phytotoxic effects of aqueous extracts and essential oils derived from southern marigold (Tagetes minuta L.), were studied using test species bioassays. The phytotoxic potential of the chemical substances was evaluated on callus induction and growth of four receptor plant species: Oryza sativa (Dongjinbyeo), Brassica campestris subsp. napus var. pekinensis, Raphanus sativus var. acanthiformis, and Sesamum indicum (Ansanggae). The receptor plants were grown on MS (Murashige & Skoog) media supplemented with 2 mg/litre 2,4-D and 0.5 mg/litre kinetin containing aqueous extract and essential oil extracted from T. minuta. † Corresponding author. H01050; published 25 September 2002 Received 30 October 2001; accepted 2 July 2002The induction of calluses was significantly inhibited proportional to the concentrations of aqueous extract and essential oil used. The callus induction and growth were rapidly decreased in 30 and 50% of original aqueous extract, and in 10 and 20 \i\ essential oil. The allelopathic potential of aqueous extract from T. minuta may be more than that of essential oil. It was concluded that the inhibitory allelophatic effects of marigold exhibited speciesspecific inhibitory trends.
The leaf and petiole segments of Farfugium japonicum were cultured on Murashige and Skoog (MS) or Chu N6 media with 2,4-dichlorophenoxyacetic acid (2,4-D) alone or combinations of auxins and cytokinins for direct or indirect somatic embryogenesis. Petiole expiants produced yellowish embryogenic callus within 7 weeks on MS medium with 2,4-D alone. Petiole segments were a better source for embryogenic callus than leaf expiants. The frequency of embryogwenic callus and number of somatic embryos per expiant were significantly high on the medium containing 2-5 μM 2,4-D compared to other concentrations. Direct somatic embryogenesis was observed from petiole segments on N6 medium containing 5μM a-naphthalene acetic acid (NAA) and 10 μM kinetin. When these petiole segments with embryos were transferred on MS medium containing 1 μM NAA and 2 μM kinetin, clusters of secondary embryos were formed abundantly on the base of the primary somatic embryos. These clusters subsequently produced numerous plantlets on the MS medium without plant growth regulators. The survival rate of regenerated plants was 97% after 1 month of transplantation, and they were morphologically similar to the original plant.
scite is a Brooklyn-based organization that helps researchers better discover and understand research articles through Smart Citations–citations that display the context of the citation and describe whether the article provides supporting or contrasting evidence. scite is used by students and researchers from around the world and is funded in part by the National Science Foundation and the National Institute on Drug Abuse of the National Institutes of Health.
hi@scite.ai
10624 S. Eastern Ave., Ste. A-614
Henderson, NV 89052, USA
Copyright © 2024 scite LLC. All rights reserved.
Made with 💙 for researchers
Part of the Research Solutions Family.