Background and aims:To determine actual concentration of clinically significant IgG antibodies, there is need to inactivate IgM antibodies. Aim of this study was to study effect of dithiothreitol (DTT) on ABO isoagglutinin titers performed by column agglutination technology (CAT) and conventional tube technique (CTT) and to compare results obtained by DTT treatment. Materials and methods:This was a prospective, observational study conducted from October 2018 to March 2020. All consecutive O group donors were included. All samples were consecutively tested by CTT and CAT, before and after DTT treatment (pCTT, pCAT).Results: A total of 2005 donors were included. 295 (14.71%) had inter-observer variation by CTT and 51 (2.54%) had inter-observer variation by CAT. IgG titers were found to be more than IgM titers. Reduction in IgG titers was observed with DTT. Concordance between CTT and pCAT was found to be unsatisfactory. pCTT results showed one-fold higher decrease than pCAT when compared to pre-treatment results. IgG titers for both anti-A and anti-B showed strong correlation between CTT (1+ strength) and pCAT (2+ strength). Comparison between CTT (1+) and CAT (1+, 2+, 3+), pCTT (1+) and CAT (1+, 2+, 3+), pCTT (1+) and pCAT (1+,2+, 3+) was performed using Wilcoxon signed-rank paired test for significance. For all comparisons, test was significant at 1+ and not significant at 3+ end point. Conclusion:There is significant difference between antibody titers estimated using DTT treated and untreated plasma. Use of DTT for ABO isoagglutinin titer estimation is recommended.
Background and aims: Measurement of actual concentration of IgG requires methods like heat inactivation (HI) of plasma. This study was aimed at comparing of heat treated ABO titers performed by conventional test tube technique (CTT) and column agglutination technique (CAT) with HA/SPRCA. Materials and methods:This was a prospective, observational study conducted from October 2018 to March 2020. All consecutive O group donors who gave consent for participation were included. All samples were tested by CTT and CAT before and after HI (pCTT, pCAT) and with HA/SPRCA.Results: A total of 2005 donors were included. IgG titers were found to be more than IgM titers. PCTT IgG results are similar or lower when compared to results obtained by SPRCA, while pCAT IgG titers were higher with pCAT when compared to HA/SPRCA. Results of titers obtained by pCTT were lower as compared to pCAT and HA/SPRCA, with majority giving results less than 64. Median IgG and IgM titers for both anti-A and anti-B were highest in pCAT, while median IgG and IgM anti-A and anti-B titers were similar with HA and pCTT. Conclusion:Results obtained by HA/SPRCA were closer to results obtained by pCTT, with the advantage of less time consumption, automation requiring less expertise and no inter-observer variation. Titers obtained by pCAT were higher in comparison to HA/SPRCA and pCTT results, due to high sensitivity.
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