Methicillin resistant Staphylococcus aureus is considered the multidrug resistant bacterium due to developing bio lm formation associated with antimicrobial resistance mechanisms. Therefore, inhibition of bio lm formation is an alternative therapeutic action to control MRSA infections. The present study revealed the non-antibacterial bio lm inhibitory potential of hesperidin against ATCC strain and clinical isolates of S. aureus. In addition, hesperidin treatment signi cantly impedes lipase, hemolysin, autolysin, autoaggregation and staphyloxanthin production. Reductions of staphyloxanthin production possibly increase the MRSA susceptibility rate to H 2 O 2 oxidative stress condition. In gene expression study revealed the hesperidin treatment downregulated the bio lm-associated gene (sarA), polysaccharide intracellular adhesion gene (icaA and icaD), autolysin (altA), bronectin-binding protein (fnbA and fnbB) and staphyloxanthin production (crtM). Molecular docking analysis predicted the ability of hesperidin to interact with SarA and CrtM proteins involved in bio lm formation and staphyloxanthin production in MRSA.
scite is a Brooklyn-based organization that helps researchers better discover and understand research articles through Smart Citations–citations that display the context of the citation and describe whether the article provides supporting or contrasting evidence. scite is used by students and researchers from around the world and is funded in part by the National Science Foundation and the National Institute on Drug Abuse of the National Institutes of Health.