The extensive consumption of antibiotics has undesired effects on hosts including high sensitivity, suppressing the immune system, and the allergic reactions in addition to making bacteria resistant. 1,2 The problems raised by resistant bacteria have caused several challenges in the therapeutic system. 2 Nowadays, the spread of Gram-negative pathogens resistant to drugs in hospitals has become a great problem, and is rising in many countrie. 3 Acinetobacter is one of these pathogens which are found in different wards of hospitals, especially in intensive care units (ICUs). The resistance among Acinetobacter spp. has increased dramatically in recent years and has become a global threat. 4 Acinetobacter is one important reason for pneumonia and blood infections in the ICU. 5-7 Currently, it is necessary to find some stronger and more effective antibacterial substance against multi-drug resistant bacteria. 2 From many years ago, medicinal plants have been used for the treatment of different infections. 8 Nowadays, these plants have attracted the attention researchers because of their significant attributes including their strong antibacterial property, being cheap, and accessible. 9,10 One of these medicinal plants which are local in Iran is the shallot (Allium hirtifolium) which has different therapeutic applications. 11 The Iranian shallot is a member of the Allium family and has bulbs (short stem with meaty leaves). This plant is rich in combinations of organosulfur and flavonoids, and due to these combinations it has a strong anti-oxidant and antibacterial activity. Conducted investigations have shown that the aqueous and alcoholic extracts of the stems and the leaves of this plant have a strong antibacterial property. 12-14 The nettle has a long history in treating different diseases and has been used in traditional medicine too. 15 Different studies have revealed that the nettle extract has strong antibacterial
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