The aim of the present study was to investigate the effect of wall materials composition on physicochemical characteristics of fish oil microcapsule produced by spray-drying (180 oC). Four different combination of coating materials (fish gelatin, chitosan, combination of gelatin and chitosan and a mixture of microbial transglutaminase (MTGase) with maltodextrin) were applied to two different fish oils to produce 40% solid emulsions. Scanning electron microscopy and extraction of surface and encapsulated oils revealed that fish gelatin had the highest ability of covering fish oil ; Meantime, addition of MTGase to gelatin could also increase this ability and reveled less surface oil (2.63% than 5.23% on chitosan treatment (P<0.05). Mixture of gelatin and maltodextrin with MTGase as the wall material led to a high encapsulation efficiency 88%, so was selected as the best microcapsule. However, application of chitosan with maltodextrin had lower encapsulation efficiency ~67% (P<0.05). All indices of powders (encapsulation efficiency, surface morphology and particle size) showed that powders prepared from gelatin and gelatin with MTGase increased efficiency of encapsulation and will increase stability of the microcapsules powders.