A rare subset of HIV lymphoma, known as primary effusion lymphoma (PEL), is a high-grade tumour carrying human herpes virus 8 (HHV-8). Very little is known about genomic aberration in PEL, and only a few HIV-negative PEL have been reported. Here we report the results of chromosomal analysis and comparative genomic hybridisation (CGH) conducted to detect regions of gain and loss, in five HIV-negative Japanese cases of HHV-8-negative PEL. All patients except one (35-year-old female) were elderly men and the morphologic examination showed large cell type. PEL expressed B-cell-associated and activation-associated antigens, and exhibited clonal immunoglobulin genes. No HHV-8 was detected in all four examined cases, but Epstein-Barr virus (EBV) was detected in one case. Genomic abnormalities and aberrations were identified in all HHV-8/HIV-negative PEL. CGH studies showed gain in 19 of 24 chromosomes. Gains of 3q13-27, 8q24, 10q21-23 and Yq were detected in two of the five cases, but other gains were noted in each case. Chromosomal analysis revealed complex abnormalities both in numbers and structures. Burkitt lymphoma-associated t(8;22) was detected in one case, but +8 chromosome and c-myc amplification were detected in the other three cases by Southern blot and/or fluorecence in situ hybridization (FISH). Abnormality of chromosome 8, which associates with c-myc, was detected in four of the five HHV-8/HIV-negative PEL. However, the other common genomic abnormalities of HHV-8/HIV-negative PEL were not detected in our study, but the complex abnormalities seemed to be true rather than the usual large B-cell lymphoma. Our results suggest that multi-step genomic abnormalities might be associated in HHV-8/HIV-negative PEL tumorigenesis.
A method of measurement of solids holdup in a three-phase reactor by analyzing the shape and the phase lag or lead of an ultrasonic wave has been proposed. The solids holdup in a solids-dilute three-phase fluidized bed could be measured without being affected by the presence of gas bubbles. The experimental values of solids axial dispersivity measured by the ultrasonic technique are in reasonable agreement with an empirical correlation of previous data obtained by other methods. The possibility of the application of the ultrasonic technique to the measurement of solids holdup in a three-phase fluidized bed has been suggested.Nous proposons une mkthode de mesure de la retention des solides dans un reacteur triphasique en analysant la forme et le retard ou I'avance de phase d'une onde ultrasonore. La retention des solides dans un lit fluidis6 de solides triphasique dilut pourrait &re mesuree sans Ctre modifike par la presence des bulles de gaz. Les valeurs expkrimentales de la dispersivitk axiale des solides par la technique ultrasonore montrent un bon accord avec la corrklation empirique des donnkes antkrieures obtenues avec d'autres methodes. On suggkre d'appliquer la technique ultrasonore pour mesurer la retention des solides dans les lits fluidisks triphasiques.
The ultrasonic method developed to measure the dispersed phase holdups in dispersion systems is based on the fact that the velocity of ultrasound in the dispersion is different from that in the liquid. The relationships between velocity differences in the term of transmission time differences of ultrasound and gas holdup in a gas‐liquid system and solid holdup in a liquid‐solid system were derived. The holdups became linearly dependent on the time differences, which is in agreement with the experimental data. Based on those relations, a model for simultaneous measurement of gas and solid holdups in three‐phase systems is proposed. The model permits measurement of the dispersed phase holdups by measuring the transmission time of ultrasound transmitted through the dispersions in two frequencies. It allows investigation of local holdups distributions in a bubble column and in a suspended bubble column.
This paper describes a new sensor for high moisture measurement in producing green tea. The sensor is based on the microwave transmission technique. It uses a microstripline, on which tea leaves under test are overlaid. A microwave signal at 3 GHz was transmitted along the line, and attenuation and phase shift of the wave were measured. The moisture content of the leaves was calculated by a new idea of using the ratio of two signals corresponding to the attenuation and the phase shift. The sensor measured a moisture content range from 155 to 320% on a dry basis.
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