Background: Leukemia is a malignant blood disease caused by the overproduction of a large number of immature blood cells that enter the peripheral blood. Because of the side effects associating the chemotherapy of leukemia, the identification of medicinal herbs, therefore, remains to be an attractive goal to treat leukemia.

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Matrix metalloproteinases (MMPs) were postulated to have important implication in progression and invasiveness of many malignant disorders. On the other hand the biological role of MMP-2 in acute myeloid leukaemia (AML) is not fully clear. Serum samples from 37 adult patients with AML had been taken before chemotherapy was administered. In addition 20 out of the 37 patients were analysed again after achieving complete remission (CR). Ten samples from healthy volunteers were evaluated as the control. Total MMP-2 levels were measured using ELISA Kit obtained from R&D system. MMP-2 serum levels were significantly lower in pretreatment AML patients than that in the normal controls (p = 0.000) and in CR (p = 0.007). No significant correlations were detected between pretreatment sMMP-2 levels and FAB subtypes, peripheral blood blast cell counts, peripheral blood WBCs, bone marrow blast cell counts or blast cell distribution ratio. The prognostic value of MMP-2 was evaluated by dividing AML patients into high and low MMP-2 groups using the pretreatment median MMP-2 level of the AML group as the cut-off. The authors found that patients in the high group survived for a significantly shorter time than those patients in the lower MMP-2 group. High pretreatment levels of sMMP-2 among AML patients were associated with poor survival. Prospective studies are recommended to establish the clinical value of longitudinal sMMP-2 measurement.

In an attempt to alleviate the aflatoxicosis symptoms by broiler chicks fed on low (50 ppb) aflatoxin-contaminated diet; the dietary nutrients were elevated by 2.6% for protein, 0.54 MJ ME/kg for energy or 0.5% for each of methionine and lysine. These supplements were mixed altogether and applied as well. All the experimental diets were offered for a 6-week period followed by a 2-week period on aflatoxin--free diet without supplements as a withdrawal period. It was proved after the treatment period that the raising of energy concentration was beneficial to overcome the aflatoxin effects on the body weight, feed conversion rate, and dressing (eviscerated weight) percentage and stability of relative weights of liver and kidneys. Additionally, the supplements' mixture improved the body weight and the relative weight of the liver comparing with the chicks fed on the aflatoxin diet without additives. All of the contaminated diets, except that of the high energy, caused apparently toxic symptoms besides the internal gross pathology. The high energy diet reflected the lowest mortality rate, whereas the highest was with the amino acids supplemented one. The withdrawal period resulted in lower differences among treatments in the body weight. It improved also the dressing percentage (significantly) and to some extent the results of the post-mortem examination.