G. 2015. Broadening genetic diversity in Brassica napus canola: Development of canola-quality spring B. napus from B. napusB. oleracea var. alboglabra interspecific crosses. Can. J. Plant Sci. 95: 29Á41. The narrow genetic base in spring Brassica napus (AACC) canola is a limitation for continued improvement of this crop. This research focused on broadening of genetic diversity in spring canola by using B. oleracea (CC). Seeds of B. oleracea contain high levels of erucic acid and glucosinolates, which are undesired in canola. Therefore, inheritance of these traits and the prospect of developing spring canola with allelic diversity introgressed from B. oleracea were investigated in B. napus) B. oleracea interspecific progenies. Zero-erucic plants in F 2 generation occurred at a lower frequency than expected based on segregation involving only the C-genome erucic acid alleles. Selection in F 2 to F 3 focused on zero erucic acid, while focus in later generation was for low glucosinolate and B. napus plants. In the F 6 , 31% zero-erucic families had low glucosinolate content. Flow cytometry analysis of the F 8 families showed no significant difference from the B. napus parent. Genetic diversity analysis by using simple sequence repeat markers from the C-genome chromosomes showed that the F 8 families received up to 54% alleles from B. oleracea. The results demonstrate the feasibility of enriching genetic diversity in B. napus canola by using B. oleracea. . et Se´guin-Swartz, G. 2015. É largissement de la diversite´ge´ne´tique du canola Brassica napus: de´veloppement de B. napus de printemps de qualite´canola a`partir de croisements interspe´cifiques B. napusB. oleracea var. alboglabra. Can. J. Plant Sci. 95: 29Á41. L'e´troite base ge´ne´tique des varie´te´s de printemps de Brassica napus (AACC) de qualite´canola entrave l'ame´lioration de cette culture. La pre´sente recherche devait e´largir la diversite´ge´ne´tique du canola de printemps en recourant a`B. oleracea (CC). Les graines de B. oleracea renferment une grande quantite´d'acide e´rucique et de glucosinolates, deux compose´s qu'on ne souhaite pas retrouver dans le canola. C'est pourquoi les auteurs ont examineĺ 'he´re´dite´de ces caracte`res et la possibilite´de trouver un canola de printemps posse´dant une diversite´alle´lique introgresse´e de B. oleracea dans la proge´niture du croisement interspe´cifique B. napus)B. oleracea. Les plants sans acide e´rucique de la F 2 sont moins fre´quents que pre´vu quand la se´gre´gation ne repose que sur les alle`les de l'acide e´rucique du ge´nome C. La se´lection de la F 2 a`la F 3 se concentrait sur l'absence d'acide e´rucique, tandis que, dans les ge´ne´rations subse´quentes, elle insistait sur la faible teneur en glucosinolates et les plants de B. napus. Dans la F 6 , 31 % de familles sans acide e´rucique pre´sentaient une faible concentration de glucosinolates. L'analyse par cytome´trie de flux des familles de la F 8 ne re´ve`le aucune distinction majeure par rapport au parent B. napus. L'analyse de la diversite´ge´...

Séguin-Swartz, G., Beckie, H. J., Warwick, S. I., Roslinsky, V., Nettleton, J. A., Johnson, E. N. and Falk, K. C. 2013. Pollen-mediated gene flow between glyphosate-resistant Brassica napus canola and B. juncea and B. carinata mustard crops under large-scale field conditions in Saskatchewan. Can. J. Plant Sci. 93: 1083–1087. A Saskatchewan study examined hybridization between two mustard (Brassica juncea and B. carinata) crops that were either adjacent to a glyphosate-resistant canola (B. napus) crop or separated by a 5-m strip. Overall, field hybridization levels, detected with glyphosate resistance and species-specific AFLP markers, were low: 0.024% and up to 400 m in the adjacent B. juncea field and 0.013% (up to 350 m) in the separated field, and 0.005% (up to 150 m) in the adjacent B. carinata field and 0.002% (up to 65 m) in the separated field. Based on fitness information under controlled conditions, the fertility of hybrid plants is expected to be low.