The present work encompasses identification and characterization of major degradation product (DP) of OSM observed in base hydrolytic stress study. The separation of DP was carried out on a non-polar stationary phase by using high-performance liquid chromatography system (HPLC). Using waters X-bridge (250 mm × 4.6 mm, 5 µm) C18 column with gradient elution program. For the characterization study, stress samples were subjected to HPLC and UPLC-QTOF-MS/MS and based on mass fragmentation pattern, plausible structure was deduced. Further, the DP was isolated using semi-preparative liquid chromatography and concentrated the fractions using lyophilization. The isolated DP was subjected to extensive 1D (1H, 13C, and DEPT-135) and 2D (COSY, HSQC and HMBC) nuclear magnetic resonance (NMR) studies to authenticate the structure. The impurity was unambiguously named as N-( 2-((2-(dimethylamino)ethyl)(methyl)amino)-4-metho-xy-5-((4-(1-methyl -1H-indol-3-yl)pyrimidin-2-yl)amino)phenyl)-3-methoxypropanamide. Additionally, the In-Silico structure activity relation (QSAR) assessed through statistical based software's DEREK Nexus TM , and MultiCASE, Case Ultra TM widely accepted and respected software's for DP and OSM.
Asteracantha longifolia (L.) Nees. (AL) a small, spiny weed found in marshy places. It is reported for its "antitumor, hypoglycemic, aphrodisiac, antibacterial, free radical scavenging and lipid peroxidation, hepatoprotective activities" 3 . Accompanying its uses for a medicinal purpose the plant is also used as a leafy vegetable in some states of India. Hence study of mineral composition of Asteracantha longifolia (L.) Nees and its bioaccessibility is crucial along with studying phytoconstituents of this plant. Nutritional food value of the plant can be accessed by in-vitro gastrointestinal simulation in the form of Bioaccessible Fraction (BF). The bioaccessibility of minerals found in the plant is not investigated thoroughly, mainly in functional foods. Therefore, in this research work we have subjected shade-dried powdered material of AL to ICP-AES qualitative and quantitative analysis. The results revels presence of the essential elements like Calcium (Ca) at 814.5 ppm with %BF 96.5, Magnesium (Mg) at 169.2 ppm with %BF 102.8 and micronutrients like Iron (Fe) at 5.3 ppm with %BF of 25.0, Manganese (Mn) at 4.1 ppm with %BF 100.9 and Zinc (Zn) at 0.6 ppm with %BF 31.6. Successively based on the findings it is concluded that AL has good nutritional value.
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