The goal of eating five servings of fruits and vegetables a day has not yet been achieved. The intake of polyphenols such as anthocyanins (ACN) could be improved by consuming smoothies and juices that are increasingly popular, especially in children; however, bioavailability data concerning food matrix effects are scarce. Thus, we conducted a randomised, cross-over, bioavailability study (n 10) to determine the bioavailability of ACN and their metabolites from an ACN-rich grape/blueberry juice (841 mg ACN/litre) and smoothie (983 mg ACN/litre) in vivo, and the uptake of a corresponding grape/blueberry extract in vitro. After the intake of beverage (0·33 litres), plasma and fractionated urine samples were collected and analysed by ultra-performance liquid chromatography coupled to MS. The most abundant ACN found in plasma and urine were malvidin and peonidin as native ACN and as glucuronidated metabolites as well as 3,4-dihydroxybenzoic acid (3,4-DHB); minor ACN (delphinidin, cyanidin and petunidin) were only detected as native glycosides. Plasma pharmacokinetics and recoveries of urinary metabolites of ACN were not different for juice or smoothie intake; however, the phenolic acid 3,4-DHB was significantly better bioavailable from juice in comparison to smoothie. In vitro data with absorptive intestinal cells indicated that despite their weak chemical stability, ACN and 3,4-DHB could be detected at the basal side in their native forms. Whether smoothies as well as juices should be recommended to increase the intake of potentially health-promoting ACN and other polyphenols requires the consideration of other ingredients such as their relatively high sugar content.
ACNs in physiological concentrations reached the serosal compartment and reduced inflammation-related parameters, which were related to the initial steps during the pathogenesis of atherosclerosis.
The combined approach provides fast screening capabilities, spatial distribution information and the possibility to quantify metabolites. Accurate mass measurements proved to be critical in order to reliably combine data from different MS techniques. Initial results on the mycotoxin deoxynivalenol (DON) in wheat seed and phospholipids in mouse brain as a model for mammalian tissue indicate a broad applicability of the presented workflow.
This in vivo study suggests that anthocyanins at physiological doses affect neither the systemic immune system, nor GALT, or MAT in healthy, unchallenged rats.
Pharmaceuticals are widely distributed in low concentrations in the water compartment. Conventional sewage treatment plants (STP) are not capable to remove pharmaceuticals to the full extent, so alternative methods such as ozonization or photooxidation are tested as additional stage. However, it has been shown that in many cases no complete mineralization is achieved during these processes and transformation products occur. Up to now only little is known about these transformation products in terms of their structure and toxicity. The antibiotic sulfathiazole (STZ) is used in veterinary medicine and it can be detected in different water matrices such as STP effluent or surface water. We tested the ability of photooxidation for degradation of STZ using a medium pressure mercury lamp. LC‐UV showed the formation of several minor compounds and one main transformation product which has been isolated subsequently. Using NMR and high resolution mass spectrometry techniques, we were able to reveal the structure of promizole, an antituberculotic, used in the 1950s. This compound showed a reduced microbial activity in Brilliant Black Reduction Maximum Residue Level test. Furthermore, there was no difference in the effects of promizole when compared to STZ on cell toxicity and cell proliferation in two murine cell lines.
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