The enzymogel nanoparticle made of a magnetic core and polymer brush shell demonstrates a novel type of remote controlled phase-boundary biocatalysis that involves remotely directed binding to and engulfing insoluble substrates, high mobility, and stability of the catalytic centers. The mobile enzymes reside in the polymer brush scaffold and shuttle between the enzymogel interior and surface of the engulfed substrate in the bioconversion process. Biocatalytic activity of the mobile enzymes is preserved in the enzymogel while the brush-like architecture favors the efficient interfacial interaction when the enzymogel spreads over the substrate and extends substantially the reaction area as compared with rigid particles.

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The environmental footprint of agricultural production can vary significantly both between countries and within a country based on regional conditions and agricultural practices. A life cycle assessment approach was used to model fossil fuel energy inputs and greenhouse gas emissions associated with the production of sugarcane in South Africa. Results were calculated for sugarcane produced in two distinct regions, the irrigated North and the non-irrigated North Coast. Regional differences also include terrain, soil, and use of mechanization. Models were adapted to estimate the impacts of burning prior to harvest, leaving a biomass mulch blanket with green cane harvesting, and increasing the level of mechanization for harvest and other field operations. Irrigation contributes to a higher energy input in the irrigated North but differences are mitigated by the lower fertilizer, agro-chemical, and diesel use per ton of sugarcane produced there. Despite higher energy inputs in the irrigated North, greenhouse gas emissions are similar for sugarcane produced in each region. Green cane harvesting reduces energy inputs and greenhouse gas emissions by 4% and 16%, respectively, in both regions. Impacts of mechanization on soil compaction and stool damage result in lower yields and proportionally higher energy inputs and greenhouse gas emissions. Results demonstrate the potential for variability in LCA results based on regional differences in production practices within a country.

Protein isolation conditions may be determined primarily through optimization of total protein yield. Improvements in protein functional properties may be achieved with a relatively small sacrifice in yield by altering isolation conditions.

No abstract