Back ground: This study aimed to investigate Staphylococcus aureus (S. aureus) in some dairy products, Genotypically and phenotypically antibiotic resistance pattern as well as to evaluate the effectiveness of lactoferrin (LF) as a bio preservative for yoghurt. Methods: A total of 150 samples from yoghurt, ice cream and Damietta cheese (50 for each) in Assiut city, Egypt. Antimicrobial susceptibility against antibiotics commonly used in human and animals was tested using the disc diffusion method, S. aureus isolates, PCR was applied on multidrug resistant (MDR) S. aureus isolates for detection of blaz, mecA and VanA genesResults: The highest prevalence S. aureus was detected in yoghurt (38%) followed by Damietta cheese (30%) and ice cream (14%). Antimicrobial susceptibility against antibiotics S. aureus isolates showed high resistance to tetracycline, penicillin, oxacillin, ampicillin, streptomycin, amoxicillin/clavulanate and neomycin, in different percentages. blaz, mecA and VanA genes were detected at 60% for blaz gene, 40% for mecA gene and 20% for vanA gene. Lactoferrin has a satisfactory antibacterial activity MIC at 20mg/ml and MLC at 40mg/ml. The results revealed that 40mg/ml LF in yoghurt could inhibit MDR S. aureus at 2nd day while, 20mg/ml at the fourth day.Conclusion: The study concluded that LF can be used as a bio preservative in yoghurt due to its highest antimicrobial activity and acceptable sensorial properties.
S taphylococcus aureus is a significant and costly public health concern since it may enter the human nourishment chain and usually causing foodborne illness (Liu et al., 2022). Milk and milk products are known to be a source of S. aureus contamination whether they are collected from dairy animals enduring mastitis or from food handlers carrying the organism because of poor individual cleanliness (Bingol et al., 2012). Moreover, the huge extent of Multidrug Resistant (MDR) S. aureus isolates may represent an open wellbeing chance due to the spread of drug-resistant zoonotic S. aureus (Gebremedhin et al., 2022).The development of such resistant strains plays a fundamental position in restorative disappointment in each human and animal disease. The uncontrolled utilization of antibiotics in human and animals, in conjugation with terrible demonstrative methods and improper endorsing by way of unfit doctors, exacerbates the problem and constitutes a top-notch mission for the avoidance and control of this pathogen (Kimang'a, 2012). So, the consumers wanted to take natural compounds, an effective and non-antibiotic antimicrobial agent. Lactoferrin (LF) proved to be the goal
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