Proteins were extracted from the deoiled seeds ofTectona grandis Linn., Fam. Verbenaceae, a quality lumber source, in aqueous solutions of various pH's or by different concentrations of NaCl at pH 8.0. Chemical analysis of isolated protein identified 15 amino acids, of which eight were essential. Gel filtration on Sephadex G‐200 revealed the presence of six components, whose molecular weights were determined by two comparable standard methods. Seven components were resolved electrophoretically (SDS‐PAG electrophoresis) and their molecular weights were found to be 118,900, 92,300, 72,400, 62,400, 43,600, 39,800 and 32,400.
Karanja seed(Pongamia glabra) oil contains toxic flavonoids including 1.25% karanjin and 0.85% pongamol. After refining the oil resembles peanut oil in composition and is free from toxic flavonoids, bitterness and unpleasant odors. Akashmoni seed(Acacia auriculaeformis) oil is rich in stearic acid (31%), and nearly two‐thirds of its glyceride is GS2 U (disaturated monounsaturated), mostly SOS (saturated‐stearic acid and unsaturated‐oleic acid). Nutritional evaluations of these two refined seed oils were carried out in rats by feeding the respective oils and peanut oil as control at 10% level in a 20% protein diet for 12 weeks. The animals fed karanja oil showed poor growth performance, altered lipid metabolism and fatty infiltration in liver. Akashmoni oil in the diet of rats did not reveal growth retardation or any abnormalities in evaluations of lipid parameters of serum and liver or histopathological findings. The results of this study indicate that refined karanja oil is toxic to rats and may not be desirable for edible purposes, while akashmoni oil may be desirable.
Trypsin and chymotrypsin inhibitor activities of defatted seed meals of akashmoni (Acacia auriculaeformis) and karanja (Pongamia glabra) were studied before and after detoxification. Protease inhibitor activities were significantly higher in the unprocessed seed meals when compared with the processed seed meals. Detoxification also improved the in vitro protein digestibilities of these seed meals significantly. Processed meals of these legume seeds, with lower protease inhibitor activities and higher protein digestibilities, could readily be used as animal feed.
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