Gunduz et al. 2005, Sonmez et al. 2005, S , ahinler and Kaftanoglu, 2005Silici and Kutluca, 2005;Uzel et al., 2005). In this study, antimicrobial and antioxidant activities of propolis samples from different regions of Turkey were examined and these effects were investigated depending to the chemical composition of ethanol extract of propolis samples.
MATERIALS AND METHODS
Propolis samples and preparation of alcohol extracts.Eight samples of propolis were collected from Denizli (P1, P5), Denizli-Sarayköy (P2A), AydIn (P2B), Denizli-Bas,karcI (P3), AydIn (P4), Konya (P6) and Tekirdag (P7) cities in spring 2004. Each sample was cut into small pieces after cooling at -20 o C, and extracted with 96% ethanol (1:10 w/v) at 37 o C for 5 days. The ethyl alcohol extracts were then filtered through a Whatman No. 1 filter paper and evaporated to dryness under vacuum. The samples were kept at -20 o C for the sample preparation to examine in GC-MS and antimicrobial activity experiments (Blonska et al. 2004). Characterisation of the chemical compounds in propolis extracts by GC-MS. About 5 mg of propolis dry residual powder was mixed with 75 µl of dry pyridine and 50 µl bis (trimethylsilyl) trifluoroacetamide (BSTFA), heated at 80 °C for 20 min and then the final supernatant was analysed by GC-MS. A GC 6890N from Hewlett-Packard (Palo Alto, CA, USA) coupled with mass detector (MS5973, Hewlett-Packard) was used for the analysis of extracted propolis samples.Abstract -Chrysin, apigenin, flavonoids, flavanones, naringenin, ethyl oleate, 3-4-dimethoxy-cinnamic acid and 9-octadecenoic acid were the predominant components of propolis samples collected from different regions of Turkey. The extracts of P3 from Denizli-Bas ,karcI, P5 from Denizli and P7 fromTekirdag had effective antibacterial activities on Gram-negatives. Chrysin, which has antibacterial activity, was found to be high concentration. The extracts of P3, P2B from AydIn and P6 from Konya had much more effective antibacterial activities on Gram-positives. The total antioxidant activity increased with the increasing amount of extracts added to linoleic acid emulsion. All doses of propolis ethanol extract displayed antioxidant activity.
