Objective: Rheumatoid Arthritis (RA) is an autoimmune and chronic inflammatory disease of unknown etiology. Killer cell immunoglobulin-like receptors are expressed on the surface of natural killer cells and CD28nullT-cells, both present in synovial membrane of RA. Therefore we evaluated the associations ofKIRgenes with RA.Methods: 16KIRgenes were genotyped in 100 healthy subjects (HS) and 100 RA patients from Western Mexico using PCR-SSP. Differences inKIRgenotypes and gene frequencies were assessed using theX2test.Results: Gene frequency ofKIR2DL3was lower in RA than in HS (p= 0.0019), whereasKIR2DL2andKIR2DS2were higher in RA than HS (p= 0.0004 andp= 0.0487, respectively). In addition were identified 38 genotypes (from G1-G38) in both studied groups, and the genotype frequencies of G1, G6 and G14 showed significant differences (p= 0.0001,p= 0.0208 andp= 0.0300, respectively).Conclusions: The presence ofKIR2DL2,KIR2DS2and absence ofKIR2DL3are associated with RA. Moreover, two genotypes BX are associated with RA. These results suggest thatKIRscan be involved in RA susceptibility.
In our results, responders and non-responders to DMARDs showed KIR2DS2 and KIR2DL2 different gene frequencies, therefore, these genes could be used as response predictors to DMARDs treatment. Thus, these genes were also associated with disease severity, as well as the treatment response possibly by the immunoregulatory function of NK cells.
Introduction. Preventive measures taken during periods of health crisis, specifically in pandemics, have consistently been associated with detrimental effects on mental health. Isolation and loneliness are indirect effects of these preventive measures. Given these premises, monitoring the behavior of the population in the face of these eventualities becomes important. Worry as an indirect measure of anxiety and stress enables one to recognize subjects who are vulnerable to phenomena of high uncertainty, since measures taken to avoid excessive contagion can have high costs for this population. This phenomenon has been consistently observed in other pandemics such as H1/N1 influenza. Objective. To determine the prevalence of worry and perceived risk of contagion in the Guadalajara population during the COVID-19 quarantine and to identify differentiating effects. Method. A total of 255 people from western Mexico (Guadalajara, Jalisco) voluntarily participated by answering the Penn State Worry Questionnaire (PSWQ) adapted to Mexican population. The average age of the respondents, aged between 18 and 70 years, was 31.71 (± 5.19). A total of 170 women and 85 men participated in the study. Results. 40.12% of the population scored high levels of worry, making them vulnerable to mental health conditions. Subjects favored the prevention of a contagion regardless of whether they were self-isolated. The only variable that had a differential effect was sex (p < .05), and there were no differences in educational attainment, occupational demandingness, and isolation between the groups. Discussion and conclusion. A preventive attitude was observed among the participants, and so it is important to implement strategies that will prevent mental health costs in those who express excessive worry to avoid saturating mental health services.
Introduction: Epstein-Barr Virus (EBV) infection prevails in underdeveloped and developing countries. The tonsils seem to be candidate replication sites for EBV and some studies have exposed a close association among viral infections and chronic tonsillitis. The objective of this study was identifying the EBV prevalence in Mexican patients who had undergone tonsillectomy because of chronic tonsillitis.
Methodology: Frozen tissues and medical records were obtained from 50 Mexican patients. DNA was extracted and subjected to PCR to amplify the EBER-2 region of EBV. Next, the patients were classified according to general and clinical characteristics searching a relation with the EBV-DNA positivity.
Results: EBV genome was detected in 46% (23/50) of the analysed tonsil tissues. Trends were found regarding the relationship of viral presence with lower values in terms of age (6.1 ± 2.8 vs 7.6 ± 3.7) , a greater degree of hypertrophy (3.5 ± 0.4 vs 3.0 ± 0.6) and an increase in the number of episodes of tonsillitis (11 ± 7.4 vs 9 ± 6.5).
Conclusions: The prevalence found of EBV-DNA positivity in tonsillar tissues from patients diagnosed with chronic tonsillitis , supports the fact that palatine tonsils can be occupied by EBV and highlights the importance of conducting future studies focused on understanding the role of the EBV infection in chronic inflammatory processes in the population involved in this study.
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