Experimental Peptides synthesis and determination of their concentrationPeptides were synthesized by Fmoc peptide chemistry using a Shimadzu PSSM-8 automated peptide synthesizer (Shimadzu, Kyoto, Japan), and purified by reverse-phase HPLC on a C18 column. The identity and purity of the peptides were confirmed by matrix-assisted laser desorption/ionization (MALDI)-TOF MS using a MicroflexAI mass spectrometer (Bruker Daltonics, Yokohama, Japan) and α-cyano-4-hydroxycinnamic acid as a matrix. Peptides with one disulfide bond were prepared by air oxidation of reduced peptides without disulfide bonds (SH type). For air oxidation, 10 -7 M peptides were suspended in 50 mM ammonium acetate buffer (pH 8.2), stirred and left at room temperature for one or two days. After oxidation, 0.4% 2012 © The Japan Society for Analytical Chemistry † To whom correspondence should be addressed. In order to elucidate the role of desorption/ionization efficiency of peptides in MALDI-MS, we focused on peptides with disulfide bonds, which form a rigid tertiary structure. We synthesized seven sets of peptides with one disulfide bond (oxytocin, somatostatin, [Arg 8 ]-vasopressin, [Arg 8 ]-vasotocin, cortistatin, melanin-concentrating hormone, urotensin II-related peptide) and five sets of peptides with two disulfide bonds (tertiapin, α-conotoxin GI, α-conotoxin ImI, α-conotoxin MI and α-conotoxin SI). Each peptide set consisted of three peptides: the oxidized form (S-S type), the reduced form (SH type), and an internal standard peptide in which all cysteine residues were substituted with alanine residues. In the case of urotensin II-related peptide, tertiapin, α-conotoxin ImI and α-conotoxin MI, the reduced form showed higher desorption/ionization efficiency than the oxidized form. In contrast, the other peptides revealed higher desorption/ionization efficiency in the oxidized form relative to the reduced form. These results imply that a rigid structure of peptides formed by disulfide bonds does not correlate with desorption/ionization efficiency in MALDI-MS.
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