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Murine monoclonal antibodies were prepared against fumonisin B1-carrier protein conjugates, such as fumonisin B1-ovalbumin and fumonisin B1-keyhole limpet hemocyanin. Five stable hybridoma clones were obtained. The response ranges of these monoclonal antibodies for fumonisin B1 were from 10 to 1000-5000 ng/ml in the competitive indirect enzyme-linked immunosorbent assay (CI-ELISA).

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I. MASUDA‐NISHIMURA, S. FUKUDA, A. SANO, K. KASAI and H. TATSUMI.2000.We have developed a sensitive bioluminescence assay for β‐galactosidase using a luminescent substrate, d‐luciferin‐O‐β‐galactopyranoside (LuGal). The detection limit for β‐galactosidase was 3 × 10−20 mol per assay, which was approximately 50‐fold more sensitive than the test using a fluorescent substrate. This assay was applied to a positive/absent (P/A) test for coliforms. Observations made after 7 h of culture followed by a 10‐min enzyme assay using LuGal were comparable to those made after a 22–24‐h culture by the current method. Therefore, the LuGal method allows a rapid P/A test for coliforms.

S . F U K U D A , H . T A T S U M I , S . I G I M I A N D S . Y A M A M O T O . 2005.Aims: To evaluate an improved bioluminescent enzyme immunoassay (BEIA) using biotinylated firefly luciferase for the rapid detection of Salmonella in naturally contaminated chicken meat samples. Methods and Results: Capture agents and lipopolysaccharide (LPS) extraction reagents for Salmonella were investigated to improve the sensitivity of the BEIA. Also, the use of Oxoid SPRINT (Simple Pre-enrichment and Rapid Isolation New Technology) as a pre-enrichment and selective medium for 26-h BEIA detection of Salmonella in chicken meat samples was examined. The use of polymyxin B as a capture agent on solid support and 3-[(3-Cholamidopropyl) dimethylammonio] propanesulfonic acid (CHAPS) for extraction of the LPS facilitated sensitive detection of Salmonella. Of 120 chicken meat samples, 25 samples were positive using the improved BEIA with the SPRINT and 25 samples were positive using the SPRINT followed by the standard isolation methods. Conclusions: The improved BEIA, in which polymxin B was used as a capture agent and CHAPS was used for extraction of the antigen, had a sensitivity of 96AE0% and a specificity of 98AE9% for the detection of Salmonella in chicken meat. Significance and Impact of the Study: The improved BEIA combined with the SPRINT medium for the detection of Salmonella in chicken meat samples produced comparable results to the culture methods in 26 h.