Under optimal conditions of growth, senescence, a terminal phase of development, sets in after a certain physiological age. It is a dynamic and closely regulated developmental process which involves an array of changes at both physiological and biochemical levels including gene expression. A large number of biotic and abiotic factors accelerate the process. Convincing evidence suggests the involvement of polyamines (PAs) and ethylene in this process. Although the biosynthetic pathways of both PAs and ethylene are interrelated, S-adenosylmethionine (SAM) being a common precursor, their physiological functions are distinct and at times antagonistic, particularly during leaf and flower senescence and also during fruit ripening. This provides an effective means for regulation of their biosynthesis and also to understand the mechanism by which the balance between the two can be established for manipulating the senescence process. The present article deals with current advances in the knowledge of the interrelationship between ethylene and PAs during senescence which may open up new vistas of investigation for the future.
To predict the effects of temperature changes on plant growth and performance, it is crucial to understand the impact of thermal history on leaf morphology, anatomy and physiology. Here, we document a comprehensive range of leaf phenotypes in 25/20°C-grown Arabidopsis thaliana plants that were shifted to 5°C for up to 2 months. When warm-grown, pre-existing (PE) leaves were exposed to cold, leaf thickness increased due to an increase in mesophyll cell size. Leaves that were entirely cold-developed (CD) were twice as thick (eight cell layers) as their warmdeveloped (WD) counterparts (six layers), and also had higher epidermal and stomatal cell densities. After 4 d of cold, PE leaves accumulated high levels of total nonstructural carbohydrates (TNC). However, glucose and starch levels declined thereafter, and after 45 d in the cold, PE leaves exhibited similar TNC to CD leaves. A similar phenomenon was observed in d
13C and a range of photosynthetic parameters. In cold-treated PE leaves, an increase in respiration (Rdark) with cold exposure time was evident when measured at 25°C but not 5°C. Cold acclimation was associated with a large increase in the ratio of leaf Rdark to photosynthesis. The data highlight the importance of understanding developmental thermal history in determining individual phenotypic traits.
The production of hydroxyapatite (HAP) composite coatings has continuously been experimented for bone tissue applications during the last decades due to its significant bioactivity and osteoconductivity. This report unravels the...
Polyacrylamide-based
hydrogels are widely used as potential candidates
for cartilage replacement. However, their bioapplicability is sternly
hampered due to their limited mechanical strength and puncture resistance.
In the present work, the strength of polyacrylamide (PAM) hydrogels
was increased using titanium oxide (TiO2) and carbon nanotubes
(CNTs) separately and a combination of TiO2 with CNTs in
a PAM matrix, which was interlinked by the bonding between nanoparticles
and polymers with the deployment of density functional theory (DFT)
approach. The synergistic effect and strong interfacial bonding of
TiO2 and CNT nanoparticles with PAM are attributed to high
compressive strength, elastic modulus (>0.43 and 2.340 MPa, respectively),
and puncture resistance (estimated using the needle insertion test)
for the PAM–TiO2–CNT hydrogel. The PAM–TiO2–CNT composite hydrogel revealed a significant self-healing
phenomenon along with a sign toward the bioactivity and cytocompatibility
by forming the apatite crystals in simulated body fluid as well as
showing a cell viability of ∼99%, respectively. Furthermore,
for new insights on interfacial bonding and structural and electronic
features involved in the hydrogels, DFT was used. The PAM–TiO2–CNT composite model, constructed by two interfaces
(PAM–TiO2 and PAM–CNT), was stabilized by
H-bonding and van der Waals-type interactions. Employing the NCI
plot, HOMO–LUMO gap, and natural population analysis tools,
the PAM–TiO2–CNT composite has been found
to be most stable. Therefore, the prepared polyacrylamide hydrogels
in combination with the TiO2 and CNT can be a remarkable
nanocomposite hydrogel for cartilage repair applications.
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