Erythropoietin (EPO), a hormone that regulates the synthesis of red blood cells, is frequently abused by athletes. Sodium dodecyl sulphate poly acrylamide gel electrophoresis (SDS-PAGE) is an essential analytical technique in all anti-doping laboratories in order to detect the abuse of EPO. An immunoaffinity purification step is now considered essential for the pre-treatment of urine samples to isolate EPO prior to gel electrophoresis. In this study, we have compared the performance of two immunoaffinity purification techniques in EPO anti-doping analysis, i.e., the anti-EPO micro well plate and anti-EPO monolith column. The anti-EPO monolith column is efficient in removing undesirable proteins except for the Bovine Serum Albumin (BSA) as seen on SDS-PAGE. The BSA was eventually removed from the protocol and the undesirable protein band was eliminated without affecting the performance of the method. Throughout the study, the anti-EPO monolith column emerged as a better option, as it provided a higher sensitivity and higher throughput analysis when compared to the anti-EPO micro well plate. The anti-EPO monolith column has shown consistent results with the EPO recovery rate of 72%, while the limit of detection is as low as 0.5 mIU mLG 1 .

The prevalence of drug abuse is quite high in Indonesia in the land transportation sector, including public transportation. Amphetamines are among the most widely used drugs in the community which are included in the Psychotropic class II. Amphetamines are also a group of drugs that stimulate the central nervous system, which can affect the brain's cortex to increase mental activity. Amphetamines can trigger the release of several neurotransmitters in the body, such as dopamine, norepinephrine, and serQotonin. The increase caused by these neurotransmitters can increase energy stimulation, increase physical endurance, motor activity, and cause a feeling of pleasure. All organs in the body work harder, so users feel more focused, empowered, confident and able to think quickly. The effect of using amphetamine is that it can eliminate fatigue and drowsiness. The aim of the study was to determine whether the content of amphetamine compounds was present in the urine samples of public transportation drivers at the Horas Pematangsiantar Tax. The sampling location was carried out at the Pematangsiantar Horas Tax while the research location was carried out at the Simpang Bah Jambi Health Center. The type of research used is qualitative data research which is described descriptively using a stick test tool with the Immunoassay method which was carried out in May 2023. The examination results of 50 samples were negative. The conclusion based on the results of the examination was that there was no amphetamine content or negative results in the urine of public transportation drivers at the Horas Pematangsiantar Tax.