Protein kinase CK2 plays a critical role in cell growth, proliferation, and suppression of cell death. CK2 is overexpressed, especially in the nuclear compartment, in the majority of cancers, including prostate cancer (PCa). CK2-mediated activation of transcription factor nuclear factor kappa B (NF-κB) p65 is a key step in cellular proliferation, resulting in translocation of NF-κB p65 from the cytoplasm to the nucleus. As CK2 expression and activity are also elevated in benign prostatic hyperplasia (BPH), we sought to increase the knowledge of CK2 function in benign and malignant prostate by examination of the relationships between nuclear CK2 and nuclear NF-κB p65 protein expression. The expression level and localization of CK2α and NF-κB p65 proteins in PCa and BPH tissue specimens was determined. Nuclear CK2α and NF-κB p65 protein levels are significantly higher in PCa compared with BPH, and these proteins are positively correlated with each other in both diseases. Nuclear NF-κB p65 levels correlated with Ki-67 or with cytoplasmic NF-κB p65 expression in BPH, but not in PCa. The findings provide information that combined analysis of CK2α and NF-κB p65 expression in prostate specimens relates to the disease status. Increased nuclear NF-κB p65 expression levels in PCa specifically related to nuclear CK2α levels, indicating a possible CK2-dependent relationship in malignancy. In contrast, nuclear NF-κB p65 protein levels related to both Ki-67 and cytoplasmic NF-κB p65 levels exclusively in BPH, suggesting a potential separate impact for NF-κB p65 function in proliferation for benign disease as opposed to malignant disease.
Objective: To compare the risk factors with estrogen receptor (ER), progesterone receptor (PR), human epidermal growth factor receptor 2 (HER2) status of breast cancer patients.
Study Design: Cross sectional comparative study.
Place and Duration of Study: This study was carried out in Multidisciplinary Lab-1 of Army Medical College,National University of Medical Sciences, Rawalpindi, in collaboration with Department of Pathology of ArmyMedical College, NUMS Rawalpindi, from Jan 2019 to Jan 2020.
Methodology: A total of 50 individuals including radiologically diagnosed cases of breast cancer with differentstages and 10 healthy controls without cancer. Patients with any co-morbidity were excluded. Participant’ssample was collected and subjected to ER, PR and HER2 estimation. Other factors i.e. age, gender, marital status, breast feeding, menopause status, side of the breast affected were all taken into consideration.
Results: Patients characteristics showed that the mean age, marital and menopause status were linked with breast cancer. The data showed that hormone receptors i.e., ER (p=0.0001), PR (p=0.0002) and HER2 (p=0.0001) were positive among most of the cancer patients as compared to the healthy subjects without cancer. There were no association found between age and hormone receptors. Marital status, breast feeding, menopause and side of breasts involved also had no association with hormone receptors.
Conclusion: No significant association found between risk factors and hormone recpetors status of breast cancer patients in our population.
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