The present study was conducted to monitor the prevalence of salmonellae in fresh water fish in Gharbia governorate, Egypt. A total of 120 random samples of fresh water fish (Tilapia niloticus, Mugil cephalous and Clarias lazera, 40 of each) were analyzed bacteriologically for the presence of salmonella pathogens and were further identified using biochemical and serological tests. Positive samples were confirmed by polymerase chain reaction (PCR) through detection of common virulent genes invasion A (invA) and hyper-invasive locus (hilA). The obtained biochemical and serological results revealed presence of seven different serotypes including S. typhimurium which was the most frequent one followed by S.
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